首次检测到并完成了一种自然感染胖大海的新型马铃薯 Y 病毒的全基因组序列。

First detection and complete genome sequence of a new potexvirus naturally infecting Adenium obesum.

机构信息

eResearch, Research Infrastructure, Academic Division, Queensland University of Technology, Brisbane, QLD, 4000, Australia.

Department of Agriculture, Forestry and Fisheries, PO Box 858, Canberra, ACT, 2061, Australia.

出版信息

Arch Virol. 2023 Sep 7;168(10):244. doi: 10.1007/s00705-023-05871-x.

DOI:10.1007/s00705-023-05871-x

PMID:37676508

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10484807/

Abstract

Here, we report the detection and complete genome sequence of a novel potexvirus, tentatively named "Adenium obesum virus X" (AobVX), isolated from Adenium obesum, that was sent for virus screening at Australian Government post-entry quarantine (PEQ) facilities after being imported into Australia from China. The AobVX genome is 6781 nucleotides in length excluding the poly(A) tail and is predicted to encode conserved potexvirus proteins and sequence motifs across five open reading frames. The RNA-dependent RNA polymerase of this virus shares the highest amino acid sequence similarity with that of nerine potexvirus 1 (58.7% identity) and nerine virus X (58.58% identity). This is the first report of a positive-sense single-stranded RNA virus in A. obesum related to members of the genus Potexvirus in the family Alphaflexiviridae.

摘要

在这里，我们报告了一种新型马铃薯 Y 病毒的检测和全基因组序列，暂命名为“沙漠牡丹病毒 X”（AobVX），该病毒从中国进口到澳大利亚后，在澳大利亚政府入境后检疫（PEQ）设施中进行病毒筛选时被发现。AobVX 基因组全长 6781 个核苷酸，不包括 poly(A) 尾，预计编码保守的马铃薯 Y 病毒蛋白和横跨五个开放阅读框的序列基序。该病毒的 RNA 依赖的 RNA 聚合酶与那雪花莲马铃薯 Y 病毒 1（58.7%同一性）和雪花莲病毒 X（58.58%同一性）的氨基酸序列相似度最高。这是首次在与 Alphaflexiviridae 科马铃薯 Y 病毒属相关的沙漠牡丹属植物中发现正链单链 RNA 病毒。