Hanson S R, Paxton L D, Harker L A
Arteriosclerosis. 1986 Sep-Oct;6(5):511-8. doi: 10.1161/01.atv.6.5.511.
To measure the rate, extent, and time course of arterial mural thrombus formation in vivo and to assess the effects of antiplatelet therapy in that setting, we have studied autologous 111In-platelet deposition induced by experimental iliac artery aneurysms in baboons. Scintillation camera imaging analyses were performed at 1, 24, 48, and 72 hours after implantation of the device. Correction for tissue attenuation was determined by using a small, comparably located 111In source implanted at the time of surgery. In five animals, 111In-platelet activity accumulated progressively after device implantation, reaching a maximum after the third day. Repeat image analysis carried out 2 weeks after the surgical procedure also showed progressive accumulation of 111In-platelets over 3 days but at markedly reduced amounts as compared with the initial study. In five additional animals, treatment with a combination of aspirin and dipyridamole begun 1 hour after surgical implantation reduced 111In-platelet deposition to negligible levels by the third day. Although platelet survival time was shortened and platelet turnover was reciprocally increased in all operated animals, platelet survival and turnover were not affected by antiplatelet therapy. We conclude that, in contrast to platelet survival and turnover measurements, 111In-platelet imaging is a reliable and sensitive method for localizing and quantifying focal arterial thrombi and for assessing the effects of antiplatelet therapy.
为了测量体内动脉壁血栓形成的速率、程度和时间进程，并评估在这种情况下抗血小板治疗的效果，我们研究了狒狒实验性髂动脉瘤诱导的自体铟-111标记血小板沉积情况。在植入装置后的1、24、48和72小时进行闪烁相机成像分析。通过使用手术时植入的位置相对较小的铟-111源来确定组织衰减校正。在5只动物中，植入装置后铟-111标记血小板活性逐渐积累，在第三天达到最大值。手术2周后进行的重复图像分析也显示铟-111标记血小板在3天内逐渐积累，但与初始研究相比数量明显减少。在另外5只动物中，手术植入后1小时开始联合使用阿司匹林和双嘧达莫治疗，到第三天时铟-111标记血小板沉积减少到可忽略不计的水平。尽管在所有手术动物中血小板存活时间缩短且血小板周转率相应增加，但血小板存活和周转率不受抗血小板治疗的影响。我们得出结论，与血小板存活和周转率测量不同，铟-111标记血小板成像对于局部动脉血栓的定位和定量以及评估抗血小板治疗的效果是一种可靠且敏感的方法。
