三维支架中的干细胞培养和分化。
Stem cell culture and differentiation in 3-D scaffolds.
机构信息
Department of Regeneration Science and Engineering, Institute for Life and Medical Sciences, Kyoto University, Kawara-cho, Shogoin, Sakyo-ku, Kyoto, Japan.
Division of Materials Design, Faculty of Materials Science and Engineering, Warsaw University of Technology, Woloska Street, Warsaw, Poland.
出版信息
Prog Mol Biol Transl Sci. 2023;199:109-127. doi: 10.1016/bs.pmbts.2023.04.009. Epub 2023 Jul 26.
Conventional two-dimensional (2-D) cultivation are easy to utilize for human pluripotent stem (hPS) cell cultivation in standard techniques and are important for analysis or development of the signal pathways to keep pluripotent state of hPS cells cultivated on 2-D cell culture materials. However, the most efficient protocol to prepare hPS cells is the cell culture in a three dimensional (3-D) cultivation unit because huge numbers of hPS cells should be utilized in clinical treatment. Some 3-D cultivation strategies for hPS cells are considered: (a) microencapsulated cell cultivation in suspended hydrogels, (b) cell cultivation on microcarriers (MCs), (c) cell cultivation on self-aggregated spheroid [cell aggregates; embryoid bodies (EBs) and organoids], (d) cell cultivation on microfibers or nanofibers, and (e) cell cultivation in macroporous scaffolds. These cultivation ways are described in this chapter.
传统的二维（2-D）培养方法易于利用标准技术培养人多能干细胞（hPS），对于分析或开发信号通路以保持 hPS 细胞在二维细胞培养材料上的多能状态非常重要。然而，制备 hPS 细胞最有效的方案是在三维（3-D）培养单元中进行细胞培养，因为在临床治疗中需要大量的 hPS 细胞。考虑了一些用于 hPS 细胞的 3-D 培养策略：（a）悬浮水凝胶中微囊化细胞培养，（b）微载体（MC）上的细胞培养，（c）自聚集球体[细胞聚集体；胚状体（EBs）和类器官]上的细胞培养，（d）微纤维或纳米纤维上的细胞培养，以及（e）大孔支架上的细胞培养。本章将对这些培养方法进行描述。
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