Liu Xu, Lv Shouyin, Kan Wenjiao, Fan Boxi, Shao Bo
Department of Stomatology, Baoding First Central Hospital, 320 Great Wall North Street, Baoding 071000, Hebei, China.
Department of Stomatology, Inner Mongolia Autonomous Region People's Hospital, 20 Zhaowuda Road, Huhhot 010017, Inner Mongolia, China.
Br J Oral Maxillofac Surg. 2023 Oct;61(8):527-533. doi: 10.1016/j.bjoms.2023.07.016. Epub 2023 Aug 10.
This study aimed to assess effects of 3-dimensionally (3D) printed poly-d,l-lactin (PDLLA) on human alveolar bone-derived mesenchymal stem cell (h-ABMSC) osteogenic proliferation and differentiation. Human ABMSCs were cultured and identified using flow cytometry and morphological analysis. Control and PDLLA experimental groups were assessed using a Cell Counting Kit-8 (CCK-8) to detect cellular cytotoxicity and proliferative activity. Real-time quantitative polymerase chain reaction was used to determine expression levels of osteogenesis genes including alkaline phosphatase (ALP), Runt-related transcription factor 2 (Runx-2), osteopontin (OPN), and osteocalcin (OCN). The results showed that h-ABMSCs were successfully cultured and revealed by microscopic observation. Human ABMSCs were spindle-shaped, with clustered and fish-like primary cells. Cell surface markers were negative for CD34 and positive for CD44 and CD90. PDLLA had no cytotoxicity. Human ABMSCs proliferated normally, and osteogenic differentiation of the cells was observed on the surface of PDLLA. Cellular proliferative activity and expression levels of osteogenesis-related genes of PDLLA and control groups showed no significant difference, including ALP, Runx-2, OPN, and OCN. These results suggest that 3D-printed PDLLA has good cell compatibility and biological activity.
本研究旨在评估三维（3D）打印的聚-d，l-乳酸（PDLLA）对人牙槽骨来源的间充质干细胞（h-ABMSC）成骨增殖和分化的影响。使用流式细胞术和形态学分析对人ABMSCs进行培养和鉴定。使用细胞计数试剂盒-8（CCK-8）评估对照组和PDLLA实验组，以检测细胞毒性和增殖活性。采用实时定量聚合酶链反应来测定成骨基因的表达水平，包括碱性磷酸酶（ALP）、 runt相关转录因子2（Runx-2）、骨桥蛋白（OPN）和骨钙素（OCN）。结果显示，h-ABMSCs成功培养，并通过显微镜观察得以显示。人ABMSCs呈纺锤形，原代细胞呈簇状且类似鱼形。细胞表面标志物CD34呈阴性，CD44和CD90呈阳性。PDLLA无细胞毒性。人ABMSCs正常增殖，且在PDLLA表面观察到细胞的成骨分化。PDLLA组和对照组的细胞增殖活性和成骨相关基因的表达水平无显著差异，包括ALP、Runx-2、OPN和OCN。这些结果表明，3D打印的PDLLA具有良好的细胞相容性和生物活性。
