Epivolve：一种定点抗体的方案。
Epivolve: A Protocol for Site-Directed Antibodies.
机构信息
Abbratech, Inc., Branford, CT, USA.
Institute for Systems Genomics, University of Connecticut, Storrs, CT, USA.
出版信息
Methods Mol Biol. 2023;2702:587-601. doi: 10.1007/978-1-0716-3381-6_29.
Researchers can often successfully generate antibodies to predicted epitopes. Especially when the epitopes are on the surface of a protein or in a hydrophilic loop. But it is difficult to direct recombinant antibodies to bind either to- or near a specific amino acid on a protein or peptide. We have developed a unique immune-targeting strategy, that we call "Epivolve," that enables us to make site-specific antibodies (Abs). Epivolve technology leverages a highly immunogenic modified amino acid that acts as a "pseudo-hapten" immuno-target and takes advantage of Ab affinity maturation technologies to make high-affinity site-specific antibodies. Epivolve functions by the evolution of an Ab paratope to either synonymous or especially non-synonymous amino acid (aa) binding. Here we describe the use of Epivolve technology in phage display and the protocols for developing site-specific antibodies.
研究人员通常可以成功地针对预测的表位生成抗体。特别是当表位位于蛋白质表面或亲水环中时。但是，很难将重组抗体定向结合到蛋白质或肽上的特定氨基酸上或附近。我们开发了一种独特的免疫靶向策略，称为“Epivolve”，使我们能够制造针对特定位置的抗体（Abs）。Epivolve 技术利用一种高度免疫原性的修饰氨基酸，作为“伪半抗原”免疫靶标，并利用 Ab 亲和力成熟技术制造高亲和力的针对特定位置的抗体。Epivolve 通过 Ab 变区对同义或特别是非同义氨基酸（aa）结合的进化起作用。在这里，我们描述了 Epivolve 技术在噬菌体展示中的应用以及开发针对特定位置的抗体的方案。
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