Abbratech, Inc., Branford, CT, USA.
Methods Mol Biol. 2023;2702:451-465. doi: 10.1007/978-1-0716-3381-6_24.
To develop reproducible results, it is critical that all reagents used in an experiment be validated in an alternative or independent method. We present two such independent methods for determining the specificity of antibodies: (1) "MILKSHAKE," which can be used to validate the liability and specificity of antibodies directed against post-translationally-modified epitopes, and (2) "Sundae," which is a more complete alanine-like scanning method that can be used to better understand the binding and bioactivity of specific residues of a protein. We apply both of these methods to the interaction between an antibody and its antigen.
为了获得可重复的结果，至关重要的是，实验中使用的所有试剂都要通过替代或独立的方法进行验证。我们提出了两种用于确定抗体特异性的独立方法：（1）“奶昔（MILKSHAKE）”，可用于验证针对翻译后修饰表位的抗体的效价和特异性；（2）“圣代（Sundae）”，这是一种更完整的丙氨酸扫描方法，可用于更好地了解蛋白质特定残基的结合和生物活性。我们将这两种方法都应用于抗体与其抗原之间的相互作用。
