Kolset S O
Biochem Biophys Res Commun. 1986 Sep 14;139(2):377-82. doi: 10.1016/s0006-291x(86)80001-8.
Human peritoneal macrophages were cultured in vitro and labeled with [35S]-sulfate. Both on day 1 and day 6 in culture the cells were found to synthesize exclusively chondroitin sulfate proteoglycan, the main part (70%) being associated with the medium after a 20 hour pulse. The glycosaminoglycan chains were found to be oversulfated both after 1 and 6 days in culture, due to the presence of disulfated disaccharide units.
人腹膜巨噬细胞在体外培养并用[35S] - 硫酸盐标记。在培养的第1天和第6天,均发现细胞仅合成硫酸软骨素蛋白聚糖,经过20小时脉冲后,主要部分(70%)与培养基相关联。由于存在双硫酸化二糖单元,在培养1天和6天后均发现糖胺聚糖链过度硫酸化。