Uhlin-Hansen L, Eskeland T, Kolset S O
Department of Biochemistry, University of Tromsø, Norway.
J Biol Chem. 1989 Sep 5;264(25):14916-22.
Proteoglycan biosynthesis was studied in human monocytes and monocyte-derived macrophages (MDM) after exposure to typical activators of the monocyte/macrophage system: interferon-gamma (IFN-gamma), lipopolysaccharide (LPS), and phorbol 12-myristate 13-acetate (PMA). By morphological examination, both monocytes and MDM were stimulated by these activators. Treatment with IFN-gamma resulted in a slight decrease in the expression of [35S]chondroitin sulfate proteoglycan (CSPG) in both monocytes and MDM, whereas LPS treatment increased the [35S]CSPG expression 1.8 and 2.2 times, respectively. PMA, in contrast, decreased the CSPG expression 0.4 times in monocytes, whereas MDM were stimulated to increase the biosynthesis 1.9 times. An increase in the sulfate density of the chondroitin sulfate chains was evident following differentiation of monocytes into MDM due to the expression of disulfated disaccharide units of the chondroitin sulfate E type (CS-E). However, monocytes exposed to PMA did also express disaccharides of the chondroitin sulfate E type. Furthermore, the expression of CS-E in MDM was increased 2 times following PMA treatment. An inactive phorbol ester, phorbol 12,13-diacetate, did not affect the expression of CS-E in either monocytes or MDM when compared with control cultures, suggesting that protein kinase C-dependent signal pathways may be involved in the regulation of sulfation of CSPG. Exposure to LPS or IFN-gamma did not lead to any changes in the sulfation of the chondroitin sulfate chains.
在人单核细胞和单核细胞衍生的巨噬细胞（MDM）暴露于单核细胞/巨噬细胞系统的典型激活剂后，研究了蛋白聚糖的生物合成：干扰素-γ（IFN-γ）、脂多糖（LPS）和佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）。通过形态学检查，单核细胞和MDM均受到这些激活剂的刺激。用IFN-γ处理导致单核细胞和MDM中[35S]硫酸软骨素蛋白聚糖（CSPG）的表达略有下降，而LPS处理分别使[35S]CSPG表达增加1.8倍和2.2倍。相比之下，PMA使单核细胞中的CSPG表达降低0.4倍，而MDM被刺激使生物合成增加1.9倍。由于硫酸软骨素E型（CS-E）的二硫酸化二糖单元的表达，单核细胞分化为MDM后，硫酸软骨素链的硫酸化密度明显增加。然而，暴露于PMA的单核细胞也表达硫酸软骨素E型的二糖。此外，PMA处理后，MDM中CS-E的表达增加了2倍。与对照培养物相比，无活性的佛波醇酯，佛波醇12,13-二乙酸酯，对单核细胞或MDM中CS-E的表达均无影响，这表明蛋白激酶C依赖性信号通路可能参与CSPG硫酸化的调节。暴露于LPS或IFN-γ不会导致硫酸软骨素链硫酸化的任何变化。
