Gu Xinping, Deng Ying, Wang Aoqi, Gan Qinhua, Xin Yi, Paithoonrangsarid Kalyanee, Lu Yandu
Single-cell BioEngineering Group, State Key Laboratory of Marine Resource Utilization in South China Sea, School of Marine Life and Aquaculture, Hainan University, Haikou, 570228, China.
Biochemical Engineering and Systems Biology Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at King Mongkut's University of Technology Thonburi, Bangkok, 10150, Thailand.
Biotechnol Biofuels Bioprod. 2023 Sep 7;16(1):133. doi: 10.1186/s13068-023-02384-2.
The use of marine microalgae in industrial systems is attractive for converting CO into value-added products using saline water and sunlight. The plant nature and demonstrated industrial potential facilitate Chlorella spp. as excellent model organisms for both basic research and commercial application. However, the transformation method has not been developed in marine Chlorella spp., thus genetic engineering is hindered in exploiting the industrial potentialities of these strains. In this study, we provided a transformation protocol for the marine Chlorella strain MEM25, which showed robust characteristics, including high production of proteins and polyunsaturated fatty acids in multiple cultivation systems over various spatial-temporal scales. We showed that transformants could be obtained in a dramatically time-saving manner (comparable to Saccharomyces cerevisiae) with four functional proteins expressed properly. The transgenes are integrated into the genome and can be successfully inherited for more than two years. The development of a marine Chlorella transformation method, in combination with the complete genome, will greatly facilitate more comprehensive mechanism studies and provide possibilities to use this species as chassis for synthetic biology to produce value-added compounds with mutual advantage in neutralization of CO in commercial scales.
在工业系统中使用海洋微藻，通过盐水和阳光将二氧化碳转化为增值产品具有吸引力。其植物特性和已展现出的工业潜力，使小球藻属成为基础研究和商业应用的优秀模式生物。然而，海洋小球藻属的转化方法尚未开发出来，因此基因工程在挖掘这些菌株的工业潜力方面受到阻碍。在本研究中，我们为海洋小球藻菌株MEM25提供了一种转化方案，该菌株表现出强大的特性，包括在多种时空尺度的多种培养系统中能高产蛋白质和多不饱和脂肪酸。我们表明，可通过大幅节省时间的方式（与酿酒酵母相当）获得转化体，且四种功能蛋白能正确表达。转基因整合到基因组中，并能成功遗传两年以上。海洋小球藻转化方法的开发，结合其完整基因组，将极大地促进更全面的机制研究，并为将该物种用作合成生物学底盘，在商业规模上以互利优势中和二氧化碳来生产增值化合物提供可能性。
