Jain Priyanshu, Minhas Amritpreet Kaur, Shukla Sadhana, Puri Munish, Barrow Colin J, Mandal Shovon
TERI Deakin Nanobiotechnology Centre, Sustainable Agriculture Division, The Energy and Resources Institute, New Delhi, India.
School of Life and Environmental Sciences, Deakin University, Geelong, VIC, Australia.
Front Bioeng Biotechnol. 2022 Mar 17;10:842797. doi: 10.3389/fbioe.2022.842797. eCollection 2022.
Marine microalgae produce a number of valuable compounds that have significant roles in the pharmaceutical, biomedical, nutraceutical, and food industries. Although there are numerous microalgal germplasms available in the marine ecosystem, only a small number of strains have been recognized for their commercial potential. In this study, several indigenous microalgal strains were isolated from the coast of the Arabian Sea for exploring the presence and production of high-value compounds such as polyunsaturated fatty acids (PUFAs). PUFAs are essential fatty acids with multiple health benefits. Based on their high PUFA content, two isolated strains were identified by ITS sequencing and selected for further studies to enhance PUFAs. From molecular analysis, it was found both the strains were green microalgae: one of them was a sp., while the other was a sp. The two isolated strains, together with a control strain known for yielding high levels of PUFAs, , were grown in three different nutrient media for PUFA augmentation. The relative content of α-linolenic acid (ALA) as a percentage of total fatty acids reached a maximum of 50, 36, and 50%, respectively, in sp., sp., and . To the best of our knowledge, this is the first study in exploring fatty acids in sp. The obtained results showed a higher PUFA content, particularly α-linolenic acid at low nutrients in media.
海洋微藻产生许多有价值的化合物，这些化合物在制药、生物医学、营养保健品和食品工业中发挥着重要作用。尽管海洋生态系统中有大量的微藻种质，但只有少数菌株因其商业潜力而得到认可。在本研究中，从阿拉伯海沿岸分离出几种本土微藻菌株，以探索多不饱和脂肪酸（PUFAs）等高价值化合物的存在和生产情况。PUFAs是具有多种健康益处的必需脂肪酸。基于它们较高的PUFA含量，通过ITS测序鉴定出两个分离菌株，并选择它们进行进一步研究以提高PUFAs的产量。通过分子分析发现，这两个菌株都是绿色微藻：其中一个是 属的一种，而另一个是 属的一种。将这两个分离菌株与一个以高产PUFAs而闻名的对照菌株 一起，在三种不同的营养培养基中培养以增加PUFAs的产量。在 属的一种、 属的一种和 中，α-亚麻酸（ALA）占总脂肪酸的相对含量分别最高达到50%、36%和50%。据我们所知，这是首次对 属的一种中的脂肪酸进行探索的研究。所得结果表明，在低营养培养基中，PUFA含量较高，尤其是α-亚麻酸。
