Day M, Trowbridge C G
Biochem Biophys Res Commun. 1986 Sep 30;139(3):1164-8. doi: 10.1016/s0006-291x(86)80299-6.
The formation of complexes between the self-complementary ribo-dinucleoside monophosphate CpG and ethidium ion is observed by use of an ethidium ion selective electrode. The ratio of total CpG to total ethidium was varied from 50:1 to .4:1, with CpG concentrations ranging from 0.2 to 1.1 mM. Scatchard plots show that the system is strongly cooperative with respect to ethidium ion; cooperativity with respect to dinucleoside has been previously reported (Krugh, T.R., Wittlin, F.N., and Cramer, S.P. (1975) Biopolymers 14,197-210). Cooperative behavior with respect to ethidium ion implies the existence of complexes containing at least two molecules of ethidium ion in combination with one or more CpG molecules.
通过使用乙锭离子选择性电极观察到自互补核糖二磷酸单酯CpG与乙锭离子之间复合物的形成。总CpG与总乙锭的比例从50:1变化到0.4:1，CpG浓度范围为0.2至1.1 mM。Scatchard图表明该系统对乙锭离子具有强烈的协同作用；先前已报道了对二核苷的协同作用（Krugh，T.R.，Wittlin，F.N.和Cramer，S.P.（1975年）《生物聚合物》14,197 - 210）。对乙锭离子的协同行为意味着存在至少包含两个乙锭离子分子与一个或多个CpG分子结合的复合物。
