Department of Dermatology, Shaanxi Provincial Hospital of traditional Chinese Medicine, Xi'an 710003, China.
Department of Dermatology, Xiyuan Hospital China Academy of Chinese Medical Sciences, Beijing 100193, China.
J Tradit Chin Med. 2023 Oct;43(5):887-896. doi: 10.19852/j.cnki.jtcm.20230802.003.
To investigate the efficacy of Zhenxin Anshen formula (, ZXAS) on atopic dermatitis (AD) by transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) signalling pathway in mice and .
AD-like lesions were induced by 1-chloro-2,4-dinitrobenzene (DNCB) to the shaved dorsal skin of BALB/c mice. BALB/c mice were divided into five groups: normal control, model control, cetirizine, low-, medium-, and high-dose of ZXAS. After ZXAS in-tervention, the skin lesions and blood samples were collected for hematoxylin and eosin-stained and measuring the concentrations of inflammatory cytokines. Immun-oglobulin E (IgE), interleukin (IL)-4, IL-5, IL-13, and thymic stromal lymphopoietin (TSLP) were de-tected by Enzyme-linked immunosorbent assay (ELISA). The spinal cords were collected for measuring the expression of gastrin-releasing peptide receptor (GRPR), TRPV1, and TRPA1 by using immunohistochemistry, western blotting, and quantitative real-time polymerase chain reaction (qRT-PCR) analyses. In addition, 3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, ELISA, and Western blotting were conducted for analysis of primary dorsal root ganglia (DRG) neurons .
ZXAS treatment improved DNCB-induced AD-like lesions through reducing dermatitis score, number of scratching and epidermal thickness, accompanied by the de-creased IgE and Th2 inflammatory cytokines. ZXAS also supressed the mRNA and protein expression of GRPR, TRPV1, and TRPA1 in the spinal cord. The medicated sera of ZXAS decreased capsaicin-induced Ca influx and downregulated the expression of TRPV1, TRPA1, and phospholipase C in DRG neurons.
The therapeutic effect of ZXAS on AD may be related to the regulation of TRPV1 and TRPA1 and inhibition of Ca2+ signals in neurons.
通过瞬时受体电位香草醛 1（TRPV1）和瞬时受体电位锚蛋白 1（TRPA1）信号通路，研究镇心安神方（ZXAS）对小鼠变应性皮肤病（AD）的疗效。
用 1-氯-2,4-二硝基苯（DNCB）处理 shaved 背部皮肤诱导 AD 样病变。BALB/c 小鼠分为五组：正常对照组、模型对照组、西替利嗪、ZXAS 低、中、高剂量组。ZXAS 干预后，采集皮肤病变和血液样本，进行苏木精和伊红染色，并测量炎症细胞因子的浓度。酶联免疫吸附试验（ELISA）检测免疫球蛋白 E（IgE）、白细胞介素（IL）-4、IL-5、IL-13 和胸腺基质淋巴细胞生成素（TSLP）。免疫组化、Western blot 和实时定量聚合酶链反应（qRT-PCR）分析测量脊髓胃泌素释放肽受体（GRPR）、TRPV1 和 TRPA1 的表达。此外，还进行了 3-（4,5-二甲基噻唑基-2）-2,5-二苯基四氮唑溴盐（MTT）测定、流式细胞术、ELISA 和 Western blot 分析原代背根神经节（DRG）神经元。
ZXAS 治疗通过降低皮炎评分、搔抓次数和表皮厚度改善 DNCB 诱导的 AD 样病变，同时降低 IgE 和 Th2 炎症细胞因子。ZXAS 还抑制脊髓中 GRPR、TRPV1 和 TRPA1 的 mRNA 和蛋白表达。ZXAS 的药物血清降低了辣椒素诱导的 Ca2+内流，并下调了 DRG 神经元中 TRPV1、TRPA1 和磷脂酶 C 的表达。
ZXAS 治疗 AD 的疗效可能与调节 TRPV1 和 TRPA1 以及抑制神经元中的 Ca2+信号有关。
