Mao Kai-Kai, Li Hong-Ran, Zhu Jing-Yun, Jin Ming-Hui, Wang Peng, Peng Yan, Xiao Yu-Tao
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
Front Physiol. 2023 Aug 23;14:1254765. doi: 10.3389/fphys.2023.1254765. eCollection 2023.
The development of insecticide resistance in populations is a serious threat to the crop industry. Given the spread of invasive resistant populations, prospective monitoring should be accelerated, and the development of diagnostic tools for rapid and accurate assessments of insecticide resistance is essential. First, the discriminating dose and diagnostic time of the kit were determined by the glass vial method based on a susceptible strain. Then, pests that were collected from field populations were used to determine their susceptibility to seven insecticides by using the diagnostic kit. Finally, the accuracy of the kit was verified based on correlation analyses and the likelihood of insecticide control failure was assessed. Here, we describe a diagnostic kit that enables the rapid detection of resistance to chlorpyrifos, bifenthrin, deltamethrin, lambda-cyhalothrin, phoxim, chlorantraniliprole and chlorfenapyr within 1-2 h in at diagnostic doses of 0.98, 0.84, 0.38, 1.64, 0.0082, 1.75 and 0.65 μg/cm, respectively. The linear equation between mortalities under diagnostic doses and actual resistance ratios measured by the diet-overlay bioassay was determined. The high correlation indicates that the insecticide resistance levels diagnosed by the kit were consistent with the results of the diet-overlay bioassay. Moreover, we found a significant negative correlation between diagnostic mortality and the likelihood of control failure for bifenthrin ( = -0.899, = 0.001), deltamethrin ( = -0.737, = 0.024) and lambda-cyhalothrin ( = -0.871, = 0.002). The insecticide resistance diagnostic kit for is a user-friendly tool (portable, short detection time). Its excellent performance qualifies the kit as a reliable screening tool for identifying effective insecticides in sustainable resistance management.
昆虫种群中抗药性的发展对农作物产业构成严重威胁。鉴于入侵性抗性种群的扩散，应加快前瞻性监测，并且开发用于快速准确评估抗药性的诊断工具至关重要。首先，基于敏感品系通过玻璃管法确定试剂盒的区分剂量和诊断时间。然后，使用从田间种群收集的害虫，通过诊断试剂盒确定它们对七种杀虫剂的敏感性。最后，基于相关性分析验证试剂盒的准确性，并评估杀虫剂防治失败的可能性。在此，我们描述了一种诊断试剂盒，它能够在1-2小时内分别以0.98、0.84、0.38、1.64、0.0082、1.75和0.65μg/cm的诊断剂量快速检测对毒死蜱、联苯菊酯、溴氰菊酯、高效氯氟氰菊酯、辛硫磷、氯虫苯甲酰胺和溴虫腈的抗性。确定了诊断剂量下的死亡率与通过饲料涂抹生物测定法测得的实际抗性比率之间的线性方程。高度相关性表明，试剂盒诊断的抗药性水平与饲料涂抹生物测定法的结果一致。此外，我们发现联苯菊酯（r = -0.899，P = 0.001）、溴氰菊酯（r = -0.737，P = 0.024）和高效氯氟氰菊酯（r = -0.871，P = 0.002）的诊断死亡率与防治失败可能性之间存在显著负相关。该杀虫剂抗药性诊断试剂盒是一种用户友好型工具（便携式、检测时间短）。其出色的性能使该试剂盒有资格作为在可持续抗性管理中识别有效杀虫剂的可靠筛选工具。
