Yang Rongchang, Egan Siobhon, Gao Huimin, Brice Belinda, Berto Bruno P
Australian National Phenome Centre, Health Futures Institute Murdoch University Perth Western Australia Australia.
Institute of Cash Crops Hebei Academiy of Agriculture and Forestry Sciences Shijiazhuang China.
Ecol Evol. 2023 Sep 5;13(9):e10505. doi: 10.1002/ece3.10505. eCollection 2023 Sep.
A new coccidian species, n. sp., from the Australian magpie (Latham, 1801) in Western Australia, is described and characterized morphologically and molecularly. Microscopic analysis of a faecal sample identified subspheroidal oocysts ( = 20), 20-22 × 18-20 (20.7 × 18.7); length/width (L/W) ratio 1.05-1.14 (1.10). Wall bi-layered, 1.0-1.3 (1.2) thick, outer layer smooth, . 2/3 of total thickness. Micropyle and oocyst residuum absent, but usually two polar granules are present. Sporocysts ( = 28) ovoidal, 12-13 × 9-11 (12.6 × 9.7); L/W ratio 1.22-1.35 (1.30). Stieda body present, flattened to half-moon-shaped, . 0.5 deep × 2.0 wide; sub-Stieda indistinct or barely discernible, . 1.0 deep × 2.5 wide; para-Stieda body absent; sporocyst residuum present, composed of granules dispersed among the sporozoites. Sporozoites vermiform, with anterior and posterior refractile bodies and nucleus. Segments of three gene (18S rRNA, 28S rRNA and COI) were sequenced and . n. sp. exhibited 99.8% genetic similarity to sp. MAH-2013a (KF648870) followed by 99.7% genetic similarity to (Yang, Brice & Ryan, 2016) (KT224380) at the 18S rRNA gene locus. It shared 97.0% genetic similarity with an unnamed sp. (AY283852) at the 28S rRNA gene locus and it also shared the highest genetic similarity of 99.8% with the unnamed sp. from an American crow (OL999120) at the COI gene locus. Based on morphological and molecular data, this isolate is a new species named as . n. sp.
描述并从形态学和分子学角度鉴定了一种来自西澳大利亚的澳大利亚喜鹊（拉瑟姆，1801年）体内的新球虫物种，暂定为新种。对一份粪便样本进行显微镜分析，发现了亚球形卵囊（n = 20），大小为20 - 22×18 - 20（20.7×18.7）；长宽比（L/W）为1.05 - 1.14（1.10）。卵囊壁双层，厚1.0 - 1.3（1.2），外层光滑，占总厚度的2/3。微孔和卵囊残体缺失，但通常有两个极粒。孢子囊（n = 28）呈卵形，12 - 13×9 - 11（12.6×9.7）；L/W比为1.22 - 1.35（1.30）。斯氏体存在，扁平至半月形，深约0.5×宽2.0；亚斯氏体不明显或几乎不可见，深约1.0×宽2.5；副斯氏体缺失；孢子囊残体存在，由分散在子孢子间的颗粒组成。子孢子呈蠕虫状，有前后折光体和细胞核。对三个基因片段（18S rRNA、28S rRNA和COI）进行了测序，该新种在18S rRNA基因位点与球虫物种MAH - 2013a（KF648870）的遗传相似性为99.8%，其次在该基因位点与（杨、布赖斯和瑞安，2016年）（KT224380）的遗传相似性为99.7%。在28S rRNA基因位点，它与一个未命名的球虫物种（AY283852）的遗传相似性为97.0%，在COI基因位点，它与来自美国乌鸦的未命名球虫物种（OL999120）的遗传相似性最高，为99.8%。基于形态学和分子数据，该分离株是一个新物种，命名为新种。
