Douvres F W
J Parasitol. 1979 Feb;65(1):79-84.
Advanced stages of Trichostrongylus axei were grown from exsheathed infective larvae in roller culture systems consisting of a complex cell-free medium, API-6, when used alone or supplemented for 8- or 21-day intervals with pepsin or reducing agents, or both. Young adult males and females were the most advanced stages attained. This stage was attained by days 18--21, when medium API-6 contained pepsin for 8 or 21 days, with or without reducing agents for the first 8 days; and by days 24--26, when medium API-6 was nonsupplemented. Trichostrongylus axei did not advance beyond early fourth stage or fourth molt when API-6 contained reducing agents for 8 or 21 days or reducing agents and pepsin for 21 days. The optimal culture system for development to young adults required medium API-6 supplemented with pepsin for 21 days and reducing agents for the first 8 days. This system produced maximal yields of less than 1% young adults in populations of about 100,000 T. axei by days 21--28.
在由复杂无细胞培养基API-6组成的滚瓶培养系统中，单独使用API-6或每隔8天或21天添加胃蛋白酶或还原剂或两者时，可从脱鞘感染性幼虫培养出艾氏毛圆线虫的晚期阶段。雌雄成虫是所达到的最晚期阶段。当培养基API-6含有胃蛋白酶8天或21天，且在前8天有或没有还原剂时，在第18 - 21天达到该阶段；当培养基API-6未添加其他成分时，在第24 - 26天达到该阶段。当API-6含有还原剂8天或21天或含有还原剂和胃蛋白酶21天时，艾氏毛圆线虫不会超过早期第四阶段或第四次蜕皮。发育至成虫的最佳培养系统需要培养基API-6添加胃蛋白酶21天，并在前8天添加还原剂。到第21 - 28天，该系统在约100,000条艾氏毛圆线虫群体中产生的成虫最大产量不到1%。
