Douvres F W, Malakatis G M
J Parasitol. 1977 Jun;63(3):520-7.
When a 70-min exsheathing process and two-step roller culture system were used, infective larvae of Ostertagia ostertagi developed in large numbers to mature adult males and females in 28 and 29 days, respectively. Females laid fertile eggs in the 2- to 16-cell stages from day 34 on. Some of the expelled eggs developed to the morula stage, in the culture fluid, by day 52. Yields of mature adults and eggs were best when cultures were started with 100,000 larvae in 100 ml of medium. The optimal culture system produced from 20% to 35% mature adults, from days 28 through 52.
当采用70分钟的去鞘过程和两步滚瓶培养系统时，奥氏奥斯特他线虫的感染性幼虫大量发育，分别在28天和29天发育为成熟的雄性和雌性成虫。从第34天起，雌性成虫产下处于2至16细胞阶段的可育卵。到第52天，一些排出的卵在培养液中发育到桑椹胚阶段。当培养开始时在100毫升培养基中接种100,000条幼虫时，成熟成虫和卵的产量最高。从第28天到第52天，最佳培养系统产生20%至35%的成熟成虫。
