Khan Z I, Dorsman W
J Parasitol. 1978 Dec;64(6):1024-7.
Successful cultivation of Trichostrongylus colubriformis from hatched first-stage to third-stage larvae was achieved in media containing NCTC 135, chick embryo extract, fetal calf serum, and either lactalbumin hydrolysate or freshly prepared baker's yeast extract. Medium Tc5, containing yeast extract, was the simplest medium with optimal results. Cultured third-stage larvae were able to produce patent infections in guinea pigs.
在含有NCTC 135、鸡胚提取物、胎牛血清以及水解乳白蛋白或新鲜制备的面包酵母提取物的培养基中，成功地将蛇形毛圆线虫从孵化的第一期幼虫培养至第三期幼虫。含有酵母提取物的Tc5培养基是效果最佳的最简单培养基。培养的第三期幼虫能够在豚鼠体内产生显性感染。
