Maeda Nabuki, Yumoto Takahiro, Xiong Geng, Hasegawa Yasushi
College of Environmental Technology, Muroran Institute of Technology, 27-1 Mizumoto, Muroran 050-8585, Japan.
Foods. 2023 Aug 27;12(17):3224. doi: 10.3390/foods12173224.
Previous studies have shown that mice fed a diet containing 1% mantle tissue exhibited decreased food consumption and led to death. Toxic substances present in the mantle tissue have been isolated and identified. In the present study, we explored the characteristics and stability of mantle tissue toxicity. The treatment of mantle tissue with 1 mM hydrochloric acid, 1 mM sodium hydroxide, 1 mM dithiothreitol, and 1 mM hydrogen peroxide followed by heating did not significantly reduce the toxicity of mantle tissue in mice. These results suggest that mantle toxins are stable in tissues, particularly when exposed to acidic conditions and digestive enzymes. We examined whether mantle tissue exhibited acute toxicity. Mice fed a diet containing 20% mantle tissue did not show a distinct increase in toxicity compared with mice fed a diet containing 1% mantle tissue, demonstrating that feeding mantle tissue does not lead to acute toxicity. Finally, mantle tissue toxicity in the small intestine was examined. Chronic feeding of mantle tissue to mice changed the color of the small intestine. Real-time polymerase chain reaction analysis revealed that mantle tissue feeding caused changes in inflammation and endoplasmic reticulum stress markers in the small intestine. These results suggest that mantle tissue feeding causes toxicity after initial damage to the small intestinal tissue.
先前的研究表明，喂食含有1%外套膜组织的饮食的小鼠食物摄入量减少并导致死亡。外套膜组织中存在的有毒物质已被分离和鉴定。在本研究中，我们探究了外套膜组织毒性的特征和稳定性。用1 mM盐酸、1 mM氢氧化钠、1 mM二硫苏糖醇和1 mM过氧化氢处理外套膜组织后加热，并未显著降低其对小鼠的毒性。这些结果表明，外套膜毒素在组织中是稳定的，尤其是在暴露于酸性条件和消化酶时。我们检测了外套膜组织是否具有急性毒性。喂食含有20%外套膜组织饮食的小鼠与喂食含有1%外套膜组织饮食的小鼠相比，毒性没有明显增加，这表明喂食外套膜组织不会导致急性毒性。最后，检测了外套膜组织在小肠中的毒性。长期给小鼠喂食外套膜组织会改变小肠的颜色。实时聚合酶链反应分析表明，喂食外套膜组织会导致小肠中炎症和内质网应激标志物的变化。这些结果表明，喂食外套膜组织在对小肠组织造成初始损伤后会导致毒性。
