Institute of Plant Protection, Henan Academy of Agricultural Sciences, Key Laboratory of Crop Pest Control of Henan Province, Key Laboratory of Crop Integrated Pest Management of the Southern of North China, Ministry of Agriculture of the People's Republic of China, Zhengzhou 450002, China.
Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Int J Mol Sci. 2023 Aug 24;24(17):13138. doi: 10.3390/ijms241713138.
is an important plant pest. Comparative feeding experiments showed that the egg production, oviposition duration and survival rate of beetles were significantly higher when they fed on elm leaves than when they fed on willow or purpus privet leaves. RNA sequencing was used to determine transcriptomic changes associated with oviposition. Comparative transcriptome analysis revealed that the beetles that fed on elm and willow had a total of 171 genes with differential expression. When the beetles fed on elm and purpus privet, 3568 genes had differential expression. The vitellogenesis, ovarian serine protease, odorant-binding proteins, acyl-CoA synthetase and follicle cell proteins were commonly upregulated genes in elm-fed beetles compared with those fed on willow/purpus privet leaves. The involvement of the follicle cell protein 3C gene in the regulation of oviposition was confirmed using RNA interference. The results provide insights into the molecular mechanisms underlying oviposition in feeding on different host plants. This study also describes a method for identifying potentially effective genes for pest control.
是一种重要的植物害虫。比较饲养实验表明， 取食榆树叶的成虫产卵量、产卵历期和存活率显著高于取食柳树或紫薇叶的成虫。使用 RNA 测序来确定与产卵相关的转录组变化。比较转录组分析显示，取食榆叶和柳叶的成虫共有 171 个基因表达差异。当取食榆叶和紫薇叶时，有 3568 个基因表达差异。与取食柳树/紫薇叶的成虫相比，在取食榆叶的成虫中，卵黄发生、卵巢丝氨酸蛋白酶、气味结合蛋白、酰基辅酶 A 合成酶和滤泡细胞蛋白通常是上调的基因。使用 RNA 干扰证实了滤泡细胞蛋白 3C 基因在产卵调控中的作用。研究结果为 取食不同寄主植物的产卵分子机制提供了线索。本研究还描述了一种鉴定潜在有效的害虫防治基因的方法。
