• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脂肪细胞衍生的旁分泌因子调节牛乳腺上皮细胞的体外发育。

Adipocyte-Derived Paracrine Factors Regulate the In Vitro Development of Bovine Mammary Epithelial Cells.

机构信息

Department of Physiological Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159b, 02-776 Warsaw, Poland.

出版信息

Int J Mol Sci. 2023 Aug 28;24(17):13348. doi: 10.3390/ijms241713348.

DOI:10.3390/ijms241713348
PMID:37686154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10487751/
Abstract

The mammary gland is composed of epithelial tissue forming ducts and lobules, and the stroma, composed of adipocytes, connective tissue, and other cell types. The stromal microenvironment regulates mammary gland development by paracrine and cell-cell interactions. In the present study, primary cultures of bovine mammary epithelial cells (bMEC) and bovine adipose-derived stem cells (bASC) subjected to adipogenic differentiation were used to investigate the influence of paracrine factors secreted by preadipocytes and adipocytes on bMEC development. Four types of conditioned media (CM) were collected from undifferentiated preadipocytes (preA) and adipocytes on days: 8, 12, 14 of differentiation. Next, bMEC were cultured for 24 h in CM and cell viability, apoptosis, migratory activity, ability to form spheroids on Matrigel, and secretory activity (alpha S1-casein concentration) were evaluated. CM derived from fully differentiated adipocytes (12 d and 14 d) significantly decreased the number of apoptotic cells in bMEC population and increased the size of spheroids formed by bMEC on Matrigel. CM collected from preadipocytes significantly enhanced bMEC's migration, and stimulated bMEC to produce alpha S1-casein, but only in the presence of prolactin. These results confirm that preadipocytes and adipocytes are important components of the stroma, providing paracrine factors that actively regulate the development of bovine mammary epithelium.

摘要

乳腺由形成导管和小叶的上皮组织以及由脂肪细胞、结缔组织和其他细胞类型组成的基质组成。基质微环境通过旁分泌和细胞-细胞相互作用调节乳腺的发育。在本研究中，使用原代培养的牛乳腺上皮细胞（bMEC）和牛脂肪来源干细胞（bASC）进行脂肪生成分化，以研究前脂肪细胞和脂肪细胞分泌的旁分泌因子对 bMEC 发育的影响。从未分化的前脂肪细胞（preA）和分化第 8、12、14 天的脂肪细胞中收集了 4 种条件培养基（CM）。然后，bMEC 在 CM 中培养 24 h，评估细胞活力、细胞凋亡、迁移活性、在 Matrigel 上形成球体的能力和分泌活性（αS1-酪蛋白浓度）。完全分化的脂肪细胞（12 d 和 14 d）产生的 CM 显著减少了 bMEC 群体中的凋亡细胞数量，并增加了 bMEC 在 Matrigel 上形成的球体大小。前脂肪细胞产生的 CM 显著增强了 bMEC 的迁移，并刺激 bMEC 产生 αS1-酪蛋白，但仅在催乳素存在的情况下。这些结果证实前脂肪细胞和脂肪细胞是基质的重要组成部分，提供旁分泌因子，积极调节牛乳腺上皮的发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/121dff94ce3b/ijms-24-13348-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/e5ef742c3ac7/ijms-24-13348-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/2a6ed6c80317/ijms-24-13348-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/e5739a86094f/ijms-24-13348-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/a5f4f66948a9/ijms-24-13348-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/4278f49ee53d/ijms-24-13348-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/d05c3032b508/ijms-24-13348-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/121dff94ce3b/ijms-24-13348-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/e5ef742c3ac7/ijms-24-13348-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/2a6ed6c80317/ijms-24-13348-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/e5739a86094f/ijms-24-13348-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/a5f4f66948a9/ijms-24-13348-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/4278f49ee53d/ijms-24-13348-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/d05c3032b508/ijms-24-13348-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8ac/10487751/121dff94ce3b/ijms-24-13348-g007.jpg

相似文献

1
Adipocyte-Derived Paracrine Factors Regulate the In Vitro Development of Bovine Mammary Epithelial Cells.脂肪细胞衍生的旁分泌因子调节牛乳腺上皮细胞的体外发育。
Int J Mol Sci. 2023 Aug 28;24(17):13348. doi: 10.3390/ijms241713348.
2
Milk protein expression and ductal morphogenesis in the mammary gland in vitro: hormone-dependent and -independent phases of adipocyte-mammary epithelial cell interaction.体外乳腺中的乳蛋白表达与导管形态发生：脂肪细胞与乳腺上皮细胞相互作用的激素依赖性和非依赖性阶段
Dev Biol. 1987 Mar;120(1):245-58. doi: 10.1016/0012-1606(87)90122-9.
3
Early soy exposure via maternal diet regulates rat mammary epithelial differentiation by paracrine signaling from stromal adipocytes.孕期饮食中早期接触大豆，通过基质脂肪细胞的旁分泌信号调节大鼠乳腺上皮分化。
J Nutr. 2009 May;139(5):945-51. doi: 10.3945/jn.108.103820. Epub 2009 Mar 25.
4
Cell-cell interactions promote mammary epithelial cell differentiation.细胞间相互作用促进乳腺上皮细胞分化。
J Cell Biol. 1985 May;100(5):1415-22. doi: 10.1083/jcb.100.5.1415.
5
Adipocyte derived paracrine mediators of mammary ductal morphogenesis controlled by retinoic acid receptors.脂肪细胞衍生的旁分泌介体控制乳腺导管形态发生，受视黄酸受体调节。
Dev Biol. 2011 Jan 15;349(2):125-36. doi: 10.1016/j.ydbio.2010.10.018. Epub 2010 Oct 23.
6
Growth hormone acts on the synthesis and secretion of alpha-casein in bovine mammary epithelial cells.生长激素作用于牛乳腺上皮细胞中α-酪蛋白的合成与分泌。
J Dairy Res. 2005 Aug;72(3):264-70. doi: 10.1017/S0022029905000889.
7
The role of native bovine alpha-lactalbumin in bovine mammary epithelial cell apoptosis and casein expression.天然牛α-乳白蛋白在牛乳腺上皮细胞凋亡和酪蛋白表达中的作用。
J Dairy Res. 2008 Aug;75(3):319-25. doi: 10.1017/S0022029908003403.
8
Seryl-tRNA synthetase-mediated essential amino acids regulate β-casein synthesis via cell proliferation and mammalian target of rapamycin (mTOR) signaling pathway in bovine mammary epithelial cells.丝氨酰-tRNA 合成酶介导的必需氨基酸通过细胞增殖和哺乳动物雷帕霉素靶蛋白（mTOR）信号通路调节奶牛乳腺上皮细胞中的β-酪蛋白合成。
J Dairy Sci. 2018 Nov;101(11):10456-10468. doi: 10.3168/jds.2018-14568. Epub 2018 Sep 13.
9
Growth hormone and lactogenic hormones can reduce the leptin mRNA expression in bovine mammary epithelial cells.生长激素和催乳激素可降低牛乳腺上皮细胞中瘦素mRNA的表达。
Domest Anim Endocrinol. 2006 Jul;31(1):88-96. doi: 10.1016/j.domaniend.2005.09.002. Epub 2005 Sep 21.
10
Selenomethionine stimulates expression of glutathione peroxidase 1 and 3 and growth of bovine mammary epithelial cells in primary culture.硒代蛋氨酸刺激原代培养的牛乳腺上皮细胞中谷胱甘肽过氧化物酶1和3的表达以及细胞生长。
J Dairy Sci. 2009 Jun;92(6):2670-83. doi: 10.3168/jds.2008-1901.

引用本文的文献

1
The rat mammary gland undergoes dynamic transcriptomic and lipidomic modifications from pre-puberty to adulthood.从青春期前到成年期，大鼠乳腺会经历动态的转录组和脂质组修饰。
Sci Rep. 2025 Apr 10;15(1):12222. doi: 10.1038/s41598-025-97532-z.
2
Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells.凯莫瑞蛋白刺激BME-UV1牛乳腺上皮细胞的分泌活性。
Int J Mol Sci. 2024 Apr 9;25(8):4147. doi: 10.3390/ijms25084147.

本文引用的文献

1
Collective cell migration is spatiotemporally regulated during mammary epithelial bifurcation.在乳腺上皮分叉过程中，细胞的集体迁移受到时空调节。
J Cell Sci. 2023 Jan 1;136(1). doi: 10.1242/jcs.259275. Epub 2023 Jan 5.
2
Physiological Media in Studies of Cell Metabolism.细胞代谢研究中的生理介质
Mol Biol. 2022;56(5):629-637. doi: 10.1134/S0026893322050077. Epub 2022 Oct 5.
3
The Mammary Gland: Basic Structure and Molecular Signaling during Development.乳腺：发育过程中的基本结构和分子信号
Int J Mol Sci. 2022 Mar 31;23(7):3883. doi: 10.3390/ijms23073883.
4
Chemerin Regulates Epithelial Barrier Function of Mammary Glands in Dairy Cows.凯莫瑞蛋白调节奶牛乳腺上皮屏障功能
Animals (Basel). 2021 Nov 9;11(11):3194. doi: 10.3390/ani11113194.
5
Mammary gland adipocytes in lactation cycle, obesity and breast cancer.哺乳期、肥胖与乳腺癌中的乳腺腺泡脂肪细胞。
Rev Endocr Metab Disord. 2021 Jun;22(2):241-255. doi: 10.1007/s11154-021-09633-5. Epub 2021 Mar 22.
6
Influence of Fibroblasts on Mammary Gland Development, Breast Cancer Microenvironment Remodeling, and Cancer Cell Dissemination.成纤维细胞对乳腺发育、乳腺癌微环境重塑及癌细胞播散的影响
Cancers (Basel). 2020 Jun 26;12(6):1697. doi: 10.3390/cancers12061697.
7
FGF signaling in mammary gland fibroblasts regulates multiple fibroblast functions and mammary epithelial morphogenesis.成纤维细胞生长因子信号在乳腺成纤维细胞中调节多种成纤维细胞功能和乳腺上皮形态发生。
Development. 2019 Dec 2;146(23):dev185306. doi: 10.1242/dev.185306.
8
Epithelial-adipocyte interactions are required for mammary gland development, but not for milk production or fertility.上皮细胞-脂肪细胞相互作用对于乳腺发育是必需的，但对于产奶或生育能力则不是必需的。
Dev Biol. 2020 Feb 15;458(2):153-163. doi: 10.1016/j.ydbio.2019.11.001. Epub 2019 Nov 4.
9
Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture.血清来源对培养中人间充质干细胞成骨分化的影响。
Int J Mol Sci. 2019 Oct 11;20(20):5051. doi: 10.3390/ijms20205051.
10
Molecular signature of the putative stem/progenitor cells committed to the development of the bovine mammary gland at puberty.青春期牛乳腺发育中假定的干/祖细胞的分子特征。
Sci Rep. 2018 Nov 1;8(1):16194. doi: 10.1038/s41598-018-34691-2.