Center of Clinical Neuroscience, Department of Neurology, University Hospital Carl Gustav Carus, University of Technology, 01307 Dresden, Germany.
Int J Mol Sci. 2023 Aug 30;24(17):13479. doi: 10.3390/ijms241713479.
Lymphocytes are key players in the pathogenesis of multiple sclerosis and a distinct target of several immunomodulatory treatment strategies. In this study, we aim to evaluate the effect of various pre-analytic conditions on immune cell counts to conclude the relevance for clinical implications. Twenty healthy donors were assessed for the effects of distinct storage temperatures and times after blood draws, different durations of tourniquet application, body positions and varying aspiration forces during blood draws. Immune cell frequencies were analyzed using multicolor flowcytometry. While storage for 24 h at 37 °C after blood draws was associated with significantly lower cell counts, different durations of tourniquet application, body positions and varying aspirations speeds did not have significant impacts on the immune cell counts. Our data suggest that immune cell counts are differently affected by pre-analytic conditions being more sensitive to storage temperature. Pre-analytic conditions should be carefully considered when interpreting the laboratory values of immune cell subpopulations.
淋巴细胞是多发性硬化症发病机制中的关键因素，也是几种免疫调节治疗策略的明确靶点。在这项研究中，我们旨在评估各种分析前条件对免疫细胞计数的影响，以得出对临床意义的相关结论。我们评估了 20 名健康供体在采血后不同的储存温度和时间、止血带使用时间长短、不同体位和采血时不同的抽吸速度对免疫细胞计数的影响。使用多色流式细胞术分析免疫细胞频率。虽然采血后在 37°C 下储存 24 小时与细胞计数显著降低相关，但止血带使用时间长短、不同体位和不同的抽吸速度对免疫细胞计数没有显著影响。我们的数据表明，免疫细胞计数受到分析前条件的影响不同，对储存温度更敏感。在解释免疫细胞亚群的实验室值时，应仔细考虑分析前条件。
