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酶介导的小麦面筋纤维形成对水解程度的调控：结构特征及作用机制分析。

Degree of Hydrolysis Regulated by Enzyme Mediation of Wheat Gluten Fibrillation: Structural Characterization and Analysis of the Mechanism of Action.

机构信息

China-Canada Joint Lab of Food Nutrition and Health (Beijing), Beijing 100048, China.

Key Laboratory of Special Food Supervision Technology for State Market Regulation, Beijing 100048, China.

出版信息

Int J Mol Sci. 2023 Aug 31;24(17):13529. doi: 10.3390/ijms241713529.

DOI:10.3390/ijms241713529
PMID:37686349
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10488075/
Abstract

The impact of different degrees of hydrolysis (DHs) on fibrillation when trypsin mediates wheat gluten (WG) fibrillation has not been thoroughly investigated. This study discussed the differences in amyloid fibrils (AFs) formed from wheat gluten peptides (WGPs) at various DH values. The results from Thioflavin T (ThT) fluorescence analysis indicated that WGPs with DH6 were able to form the most AFs. Changes in Fourier Transform Infrared (FTIR) absorption spectra and secondary structure also suggested a higher degree of fibrillation in DH6 WGPs. Analysis of surface hydrophobicity and ζ-potential showed that DH6 AFs had the highest surface hydrophobicity and the most stable water solutions. Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) images revealed the best overall morphology of DH6 AFs. These findings can offer valuable insights into the development of a standardized method for preparing wheat gluten amyloid fibrils.

摘要

不同水解度（DH）对胰蛋白酶介导的小麦面筋（WG）纤化时纤化的影响尚未得到彻底研究。本研究探讨了不同 DH 值下小麦面筋肽（WGPs）形成的淀粉样纤维（AFs）的差异。硫黄素 T（ThT）荧光分析的结果表明，DH6 的 WGPs 能够形成最多的 AFs。傅立叶变换红外（FTIR）吸收光谱和二级结构的变化也表明，DH6 WGPs 的纤化程度更高。表面疏水性和 ζ-电位分析表明，DH6 AFs 的表面疏水性最高，水溶液最稳定。扫描电子显微镜（SEM）和透射电子显微镜（TEM）图像显示了 DH6 AFs 最佳的整体形态。这些发现为开发一种标准化的小麦面筋淀粉样纤维制备方法提供了有价值的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d10d/10488075/5be11cc1d409/ijms-24-13529-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d10d/10488075/657bed5f571c/ijms-24-13529-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d10d/10488075/bad45c2a3565/ijms-24-13529-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d10d/10488075/5be11cc1d409/ijms-24-13529-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d10d/10488075/c38b73118006/ijms-24-13529-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d10d/10488075/1d9be1f0cba7/ijms-24-13529-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d10d/10488075/6029edc677a7/ijms-24-13529-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d10d/10488075/bad45c2a3565/ijms-24-13529-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d10d/10488075/5be11cc1d409/ijms-24-13529-g007.jpg

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