Wells J, Vietje B P, Wells D G, Boucher M, Bodony R P
Brain Res. 1986 Sep 24;383(1-2):333-8. doi: 10.1016/0006-8993(86)90035-1.
Embryonic mouse brain cells from the basal forebrain region were labeled in cell suspensions and transplanted into the denervated hippocampal formation of adult rats. Many labeled cells had the appearance of typical pyramidal neurons with dendrites that had both growth cones and neurites. Labeled neurons and glia were seen at several sites in the hippocampal formation. The neurons were located predominantly along the dentate granule cell layer and the pyramidal neurons had a preferred orientation of their apical dendrites toward the molecular layer. Since it was rare to see a surviving labeled neuron within the injection site, migration away from the injection site seemed important for survival of the cells. The methods used in these experiments should become an important adjunct to the methods for studying the migration, differentiation and growth of neurons and glia.
来自胚胎小鼠前脑基部区域的脑细胞在细胞悬液中被标记，并移植到成年大鼠去神经支配的海马结构中。许多标记细胞呈现出典型的锥体神经元外观，其树突既有生长锥又有神经突。在海马结构的几个部位都可见到标记的神经元和神经胶质细胞。神经元主要位于齿状颗粒细胞层，锥体神经元的顶端树突优先朝向分子层。由于在注射部位内很少见到存活的标记神经元，因此从注射部位迁移出去似乎对细胞存活很重要。这些实验中使用的方法应成为研究神经元和神经胶质细胞迁移、分化和生长方法的重要辅助手段。
