基于纳米带生物传感器的前列腺癌 microRNA 标志物检测。

Nanoribbon Biosensor-Based Detection of microRNA Markers of Prostate Cancer.

机构信息

Institute of Biomedical Chemistry (IBMC), 119121 Moscow, Russia.

Rzhanov Institute of Semiconductor Physics, Siberian Branch of Russian Academy of Sciences, 630090 Novosibirsk, Russia.

出版信息

Sensors (Basel). 2023 Aug 30;23(17):7527. doi: 10.3390/s23177527.

DOI:10.3390/s23177527

PMID:37687982

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10490786/

Abstract

Prostate cancer (PC) is one of the major causes of death among elderly men. PC is often diagnosed later in progression due to asymptomatic early stages. Early detection of PC is thus crucial for effective PC treatment. The aim of this study is the simultaneous highly sensitive detection of a palette of PC-associated microRNAs (miRNAs) in human plasma samples. With this aim, a nanoribbon biosensor system based on "silicon-on-insulator" structures (SOI-NR biosensor) has been employed. In order to provide biospecific detection of the target miRNAs, the surface of individual nanoribbons has been sensitized with DNA oligonucleotide probes (oDNA probes) complementary to the target miRNAs. The lowest concentration of nucleic acids, detectable with our biosensor, has been found to be 1.1 × 10 M. The successful detection of target miRNAs, isolated from real plasma samples of PC patients, has also been demonstrated. We believe that the development of highly sensitive nanotechnology-based biosensors for the detection of PC markers is a step towards personalized medicine.

摘要

前列腺癌 (PC) 是老年男性死亡的主要原因之一。由于早期无症状，PC 通常在进展后才被诊断出来。因此，早期发现 PC 对于有效的 PC 治疗至关重要。本研究的目的是同时高度敏感地检测人血浆样本中一系列与 PC 相关的 microRNAs (miRNAs)。为此，我们采用了基于“绝缘体上硅”结构的纳米带生物传感器系统 (SOI-NR 生物传感器)。为了提供对目标 miRNAs 的生物特异性检测，单个纳米带的表面用与目标 miRNAs 互补的 DNA 寡核苷酸探针 (oDNA 探针) 敏化。我们的生物传感器可检测到的最低核酸浓度为 1.1×10-15 M。还证明了从 PC 患者的真实血浆样本中分离出的目标 miRNAs 的成功检测。我们相信，开发用于检测 PC 标志物的高度敏感的基于纳米技术的生物传感器是迈向个性化医疗的一步。