Akbari Jonous Zahra, Shayeh Javad Shabani, Yazdian Fatemeh, Yadegari Amir, Hashemi Mohadeseh, Omidi Meisam
Department of Immunology Shahid Sadoughi University of Medical Sciences Yazd Iran.
Protein Research Center Shahid Beheshti University GC Tehran Iran.
Eng Life Sci. 2019 Feb 7;19(3):206-216. doi: 10.1002/elsc.201800093. eCollection 2019 Mar.
In this paper, a most sensitive electrochemical biosensor for detection of prostate-specific antigen (PSA) was designed. To reach the goal, a sandwich type electrode composed of reduced graphene oxide/ gold nanoparticles (GO/AuNPs), Anti-Total PSA monoclonal antibody, and anti-Free PSA antibody was assembled. The functionalized materials were thoroughly characterized by atomic force microscope spectroscopy, transmission electron microscopy, and X-ray diffraction techniques. The electrochemical properties of each of the modification step were evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. The results presented that the proposed biosensor possesses high sensitivity toward total and free PSA. Furthermore, the fabricated biosensor revealed an excellent selectivity for PSA in comparison to the other tumor markers such as BHCG, Alb, CEA, CA125, and CA19-9. The limit of detection for the proposed electrochemical biosensor was estimated to be around 0.2 and 0.07 ng/mL for total and free PSA antigen, respectively.
本文设计了一种用于检测前列腺特异性抗原（PSA）的高灵敏度电化学生物传感器。为实现这一目标，组装了一种由还原氧化石墨烯/金纳米颗粒（GO/AuNPs）、抗总PSA单克隆抗体和抗游离PSA抗体组成的夹心型电极。通过原子力显微镜光谱、透射电子显微镜和X射线衍射技术对功能化材料进行了全面表征。通过循环伏安法和电化学阻抗谱对每个修饰步骤的电化学性质进行了评估。结果表明，所提出的生物传感器对总PSA和游离PSA具有高灵敏度。此外，与其他肿瘤标志物如BHCG、Alb、CEA、CA125和CA19-9相比，所制备的生物传感器对PSA显示出优异的选择性。所提出的电化学生物传感器对总PSA和游离PSA抗原的检测限分别估计约为0.2和0.07 ng/mL。
