鉴定小鼠嗅黏膜中的免疫细胞。
Identification of Immune Cells in Murine Olfactory Mucosa.
机构信息
Department of Integrative Immunobiology, Duke University School of Medicine, Durham, NC, USA.
出版信息
Methods Mol Biol. 2023;2710:61-69. doi: 10.1007/978-1-0716-3425-7_5.
Olfactory immunology is an emerging field in the context of infectious disease and neuroimmunology, yet characterization of immune cells within the murine olfactory mucosa remains sparse. This is partially due to the difficulty in distinguishing olfactory-resident immune cells from immune cells that reside within nasal turbinate bone marrow. Using techniques like intranasal antibody labeling, we have developed methods to definitively identify olfactory immune cells via flow cytometry and immunofluorescent confocal microscopy. This protocol will describe the best practices for these methods, as well as detail how intravenous antibody labeling can be used to study the blood-olfactory barrier, an important determinant of olfactory immunity. We also include validated markers for the identification of major olfactory immune populations.
嗅觉免疫是传染病学和神经免疫学领域的一个新兴领域，但对小鼠嗅黏膜内免疫细胞的特征描述仍然很少。这部分是由于难以将嗅黏膜内的免疫细胞与鼻甲骨髓内的免疫细胞区分开来。我们使用鼻腔内抗体标记等技术，开发了通过流式细胞术和免疫荧光共聚焦显微镜来明确鉴定嗅觉免疫细胞的方法。本方案将描述这些方法的最佳实践，并详细介绍如何使用静脉内抗体标记来研究嗅血屏障，这是嗅觉免疫的一个重要决定因素。我们还包括用于鉴定主要嗅觉免疫群体的经过验证的标记物。
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