Department of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, NC, USA.
Department of Neurobiology, Duke University School of Medicine, Durham, NC, USA.
Methods Mol Biol. 2023;2710:121-129. doi: 10.1007/978-1-0716-3425-7_10.
The olfactory mucosa, lining a portion of the nasal cavity, houses the primary olfactory sensory neurons responsible for odor transduction, along with supporting cell populations. Tremendous advances have come from studying the peripheral olfactory system in animal models, especially the mouse. However, acquired human olfactory disorders lack effective therapies, and many of these conditions involve pathology in the olfactory mucosa. Thus, the ability to obtain human olfactory biopsy samples from subjects with olfactory dysfunction, or controls, may be of value. Here, we describe established techniques for collecting olfactory tissue from human subjects and preparing samples for downstream assays such as immunohistochemistry, flow cytometry, single-cell RNA-sequencing, or chromatin studies.
嗅黏膜衬覆于鼻腔的一部分，其中包含主要的嗅觉感觉神经元，负责气味转导，同时还有支持细胞群体。通过在动物模型（尤其是小鼠）中研究外周嗅觉系统，已经取得了巨大的进展。然而，获得性人类嗅觉障碍缺乏有效的治疗方法，其中许多疾病涉及嗅觉黏膜的病理变化。因此，能够从嗅觉功能障碍的受试者或对照者中获得人类嗅活检样本可能具有重要价值。在此，我们描述了从人类受试者中收集嗅组织并为下游检测（如免疫组织化学、流式细胞术、单细胞 RNA 测序或染色质研究）准备样本的既定技术。
