College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China.
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan Agricultural University, Zhengzhou, 450046, China.
Virology. 2023 Oct;587:109877. doi: 10.1016/j.virol.2023.109877. Epub 2023 Sep 4.
Porcine epidemic diarrhea virus (PEDV) has catastrophic impacts on the global pig industry. However, there remains no effective drugs for PEDV infection. Ivermectin is an FDA-approved anthelmintic drug used to treat worm infections. In this study, we reported the broad-spectrum antiviral activity of Ivermectin in vitro. Ivermectin can inhibit PEDV infections of different genotypes. Avermectin derivatives can also inhibit PEDV infections. A time of addition assay showed that Ivermectin exhibited potent anti-PEDV activity when added simultaneously with or post virus infection. Furthermore, Ivermectin significantly inhibited the late stage of viral infection by affecting viral release. RNA sequencing indicates Ivermectin induces cell cycle arrest, which may be related to its ability to inhibit viral release. Interestingly, when combined with Niclosamide, Ivermectin demonstrated an enhanced anti-PEDV effect. These findings highlight Ivermectin as a novel antiviral agent with potential for the development of drugs against PEDV infection.
猪流行性腹泻病毒（PEDV）对全球养猪业造成了灾难性的影响。然而，目前尚无针对 PEDV 感染的有效药物。伊维菌素是一种获得 FDA 批准的驱虫药，用于治疗蠕虫感染。在这项研究中，我们报道了伊维菌素在体外的广谱抗病毒活性。伊维菌素可以抑制不同基因型的 PEDV 感染。阿维菌素衍生物也能抑制 PEDV 感染。添加时间测定表明，伊维菌素在病毒感染同时或之后加入时表现出很强的抗 PEDV 活性。此外，伊维菌素通过影响病毒释放显著抑制病毒感染的晚期阶段。RNA 测序表明，伊维菌素诱导细胞周期停滞，这可能与其抑制病毒释放的能力有关。有趣的是，当与尼卡巴嗪联合使用时，伊维菌素表现出增强的抗 PEDV 作用。这些发现强调了伊维菌素作为一种新型抗病毒药物的潜力，可用于开发针对 PEDV 感染的药物。
