Sarkar A K, Roy N
Carbohydr Res. 1986 Sep 1;152:205-15. doi: 10.1016/s0008-6215(00)90300-8.
The capsular polysaccharide from Klebsiella Type 10 was found to contain D-galactose, D-glucose, D-mannose, and D-glucuronic acid in the ratios 3:1:1:1. Acid hydrolysis of the polysaccharide gave one aldobiouronic acid, one aldotriouronic acid, one aldotetraouronic acid, and two neutral disaccharides the structures of which were established. The native and carboxyl-reduced polysaccharide have been subjected as appropriate to methylation analysis and Smith degradation. Degradation of the methylated polysaccharide with base established the identity of the sugar unit preceding the glucosyluronic acid residue. The anomeric configurations of the sugar residues were determined by oxidation of the acetylated native and carboxyl-reduced polysaccharides with chromium trioxide. Based on these studies, the hexasaccharide structure 1 has been assigned to the repeating unit of the K-10 polysaccharide.
发现10型肺炎克雷伯菌的荚膜多糖含有D-半乳糖、D-葡萄糖、D-甘露糖和D-葡萄糖醛酸，其比例为3:1:1:1。该多糖的酸水解产生一种醛二糖醛酸、一种醛三糖醛酸、一种醛四糖醛酸和两种中性二糖，其结构已确定。天然和羧基还原的多糖已分别进行甲基化分析和史密斯降解。用碱降解甲基化多糖确定了葡萄糖醛酸残基之前糖单元的身份。糖残基的异头构型通过用三氧化铬氧化乙酰化的天然和羧基还原多糖来确定。基于这些研究，已将六糖结构1指定为K-10多糖的重复单元。
