Rao A S, Roy N
Carbohydr Res. 1979 Nov;76:215-24. doi: 10.1016/0008-6215(79)80020-8.
An aldotriouronic acid was isolated from the acid hydrolyzate of the polysaccharide from Klebsiella Type 61 (K-61), and its structure was established. Degradation of the permethylated polysaccharide with base established the identity of the sugar unit preceding the glucosyluronic acid residue. The modes of linkage and the sequence of different sugar residues were further confirmed by Smith degradation of K-61. The anomeric configurations of the different sugar residues were determined by oxidation of peracetylated native, and carboxyl-reduced, polysaccharides with chromium trioxide. The anomeric configuration of nonreducing D-galactosyl side-chains was further confirmed by enzymic degradation of K-61. Finally, gentiobiose was identified in the partial, acid hydrolyzate of K-61. Based on these results, the structure assigned the repeating unit of K-61 was as follows.
从61型肺炎克雷伯菌（K-61）多糖的酸水解产物中分离出一种醛糖三糖醛酸，并确定了其结构。用碱降解全甲基化多糖确定了葡萄糖醛酸残基之前糖单元的身份。通过对K-61进行Smith降解进一步证实了不同糖残基的连接方式和序列。通过用三氧化铬氧化全乙酰化的天然多糖和羧基还原多糖来确定不同糖残基的异头构型。通过对K-61进行酶促降解进一步证实了非还原型D-半乳糖基侧链的异头构型。最后，在K-61的部分酸水解产物中鉴定出龙胆二糖。基于这些结果，K-61重复单元的结构如下。
