College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
Plant Sci. 2023 Nov;336:111861. doi: 10.1016/j.plantsci.2023.111861. Epub 2023 Sep 7.
Tomato (Solanum lycopersicum) is one of the most widely cultivated vegetable crop species in the world. Tomato late blight caused by Phytophthora infestans is a severe disease, which can cause serious losses in tomato production. In this study, tomato SlbZIP68 was identified as a transcription factor that can be induced by P. infestans, salicylic acid (SA) and jasmonic acid (JA). Knockout of SlbZIP68 via clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) technology revealed a significant decrease in tomato resistance to P. infestans. Furthermore, knockout of SlbZIP68 reduced the activity of defense enzymes and increased the accumulation of reactive oxygen species (ROS). Our findings also indicated that SlbZIP68 can activate the expression of the PR genes and enhance resistance to P. infestans. In addition, SlbZIP68 can bind to the PR3 and PR5 promoters and induce gene expression, as revealed by yeast one-hybrid (Y1H) and dual-luciferase (LUC) assays. These findings not only elucidate the mechanisms of response to P. infestans but also enable targeted breeding strategies for tomato resistance to P. infestans.
番茄（Solanum lycopersicum）是世界上种植最广泛的蔬菜作物之一。由致病疫霉（Phytophthora infestans）引起的番茄晚疫病是一种严重的病害，可导致番茄生产的严重损失。在本研究中，鉴定了番茄 SlbZIP68 是一种转录因子，可被致病疫霉、水杨酸（SA）和茉莉酸（JA）诱导。通过成簇规律间隔短回文重复（CRISPR）/CRISPR 相关 9 （Cas9）技术敲除 SlbZIP68 显示番茄对致病疫霉的抗性显著降低。此外，敲除 SlbZIP68 降低了防御酶的活性并增加了活性氧物质（ROS）的积累。我们的研究结果还表明，SlbZIP68 可以激活 PR 基因的表达并增强对致病疫霉的抗性。此外，SlbZIP68 可以结合 PR3 和 PR5 启动子并诱导基因表达，酵母单杂交（Y1H）和双荧光素酶（LUC）测定证实了这一点。这些发现不仅阐明了对致病疫霉的反应机制，而且还为番茄对致病疫霉的抗性提供了靶向育种策略。
