Pan Chunyang, Li Xin, Lu Xiaoxiao, Hu Junling, Zhang Chen, Shi Lianfeng, Zhu Can, Guo Yanmei, Wang Xiaoxuan, Huang Zejun, Du Yongchen, Liu Lei, Li Junming
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Plants (Basel). 2024 Dec 21;13(24):3572. doi: 10.3390/plants13243572.
Late blight is a destructive disease affecting tomato production. The identification and characterization of resistance (R) genes are critical for the breeding of late blight-resistant cultivars. The incompletely dominant gene confers resistance against the race T of in tomatoes. Herein, we identified () as a candidate gene for through the analysis of sequences and post-inoculation expression levels of genes located within the fine mapping interval. The was subsequently validated to be a gene through targeted knockout and complementation analyses. It encodes a CC-NBS-LRR disease resistance protein, and transient expression assays conducted in the leaves of indicate that is predominantly localized within the nucleus. In comparison to its susceptible allele (), the transient expression of can elicit hypersensitive responses (HR) in , and subsequent investigations indicate that the structural integrity of the protein is likely a requirement for inducing HR in this species. Furthermore, ethylene and salicylic acid hormonal signaling pathways may mediate the transmission of the resistance signal, with - and HR-related genes potentially involved in the -mediated resistance. Our results could provide a theoretical foundation for the molecular breeding of tomato varieties resistant to late blight and offer valuable insights into elucidating the interaction mechanism between tomatoes and .
晚疫病是一种影响番茄生产的毁灭性病害。抗性（R）基因的鉴定与表征对于晚疫病抗性品种的培育至关重要。不完全显性基因赋予番茄对致病疫霉生理小种T的抗性。在此，我们通过对精细定位区间内基因的序列分析和接种后表达水平分析，鉴定出（基因名称）作为（抗性基因名称）的候选基因。随后通过靶向敲除和互补分析验证（基因名称）是一个（基因类型）基因。它编码一种CC-NBS-LRR抗病蛋白，在本氏烟草叶片中进行的瞬时表达分析表明（基因名称）主要定位于细胞核内。与其感病等位基因（等位基因名称）相比，（基因名称）的瞬时表达可在本氏烟草中引发过敏反应（HR），后续研究表明（基因名称）蛋白的结构完整性可能是该物种诱导HR所必需的。此外，乙烯和水杨酸激素信号通路可能介导（基因名称）抗性信号的传递，与（激素名称）和HR相关的基因可能参与（基因名称）介导的抗性。我们的研究结果可为番茄晚疫病抗性品种的分子育种提供理论基础，并为阐明番茄与致病疫霉之间的相互作用机制提供有价值的见解。
