Chongqing Key Laboratory of Traditional Chinese Medicine for Prevention and Cure of Metabolic Diseases, College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016, China.
Department of General Practice, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Int J Biol Macromol. 2023 Dec 31;253(Pt 2):126796. doi: 10.1016/j.ijbiomac.2023.126796. Epub 2023 Sep 7.
Diabetic patients often experience long-term risks due to chronic inflammation and delayed re-epithelialization during impaired wound healing. Although the severity of this condition is well known, the treatment options for diabetic wounds are limited. Rhubarb charcoal, a well-known traditional Chinese medicine, has been used to treat skin wounds for thousands of years. We produced a chitosan/silk fibroin sponge scaffold loaded with natural carbonized rhubarb and crosslinked it by freeze-drying to create a highly efficient RCS/SF scaffold. Rhubarb carbon and carboxymethyl chitosan exhibit antibacterial activity and promote wound healing. Owing to its 3D porous structure, this scaffold is antibacterial and pro-angiogenic. It also possesses remarkable properties, such as excellent swelling and biocompatibility. The supportive effect of carbonized rhubarb on mouse fibroblast migration is mediated at the cellular/tissue level by increased skin neovascularization and re-epithelization. Compared to the control group, RCS/SF scaffolds promoted faster healing, increased neovascularization, enhanced collagen deposition, and re-epithelialization within two weeks. The scaffold's pro-healing properties and efficient release of carbonized rhubarb, with rapid hemostatic and good sterilization effects, make it an outstanding candidate for treating diabetic wounds and novel therapeutic interventions for diabetic ulcers.
糖尿病患者在伤口愈合受损时，常因慢性炎症和上皮细胞再形成延迟而面临长期风险。尽管这种情况的严重程度众所周知，但糖尿病伤口的治疗选择有限。大黄炭作为一种著名的中药，在治疗皮肤伤口方面已有数千年的历史。我们制备了一种负载天然碳化大黄的壳聚糖/丝素纤维海绵支架，并通过冷冻干燥交联，从而构建了高效的 RCS/SF 支架。大黄炭和羧甲基壳聚糖具有抗菌活性，可促进伤口愈合。由于其 3D 多孔结构，该支架具有抗菌和促血管生成的特性。它还具有出色的性能，如优异的膨胀性和生物相容性。碳化大黄对小鼠成纤维细胞迁移的支持作用是通过增加皮肤新生血管和上皮细胞再形成在细胞/组织水平上介导的。与对照组相比，RCS/SF 支架在两周内促进更快的愈合，增加新生血管形成，增强胶原沉积和上皮细胞再形成。该支架具有促进愈合的特性，以及碳化大黄的高效释放，具有快速止血和良好的杀菌效果，使其成为治疗糖尿病伤口的优秀候选物，也是治疗糖尿病溃疡的新型治疗干预手段。
