Bioorganic Chemistry Laboratory, New Chemistry Unit, and School of Advanced Materials (SAMat), Jawaharlal Nehru Centre for Advanced Scientific Research (JNCASR), Jakkur P.O., Bengaluru, Karnataka, 560064, India.
Macromol Biosci. 2022 Sep;22(9):e2200097. doi: 10.1002/mabi.202200097. Epub 2022 Aug 9.
Wound healing is a complex process requiring multiple biological pathways and chemical responses to be activated and synchronized to recover tissue integrity. In normal physiological circumstances, the epidermal barrier restoration process through new tissue formation is highly efficient. However, increased production of reactive oxygen species (ROS), attack of pathogenic microorganisms, and high glucose level delay the normal healing process in diabetic patients. The successful treatment of diabetic wounds requires efficient strategies to control oxidative stress, promoting angiogenesis, re-epithelialization, and collagen deposition. In this study, a composite hydrogel for rapid wound healing in diabetic condition is developed by the amalgamation of hypolipidemic property of silk fibroin (SF), antioxidant property of melanin, and therapeutic effect of berberine. Studies have revealed that cross-linked mesoporous morphology of hydrogel matrix facilitates slow release of berberine to impart long-term therapeutic effects at wound site. The composite hydrogel formulation is biocompatible, stimulates effective migration of fibroblast cells, and control oxidative stress under in vitro conditions. The hydrogel served as scaffold for tissue re-epithelialization and promotes wound repair in diabetic type I Wistar rat model. This study demonstrates the ability of berberine- loaded SF-melanin composite hydrogel as a potential dressing formulation for wound healing in diabetic conditions.
伤口愈合是一个复杂的过程，需要多个生物学途径和化学反应被激活并同步，以恢复组织完整性。在正常生理情况下，通过新组织形成来恢复表皮屏障的过程非常高效。然而，在糖尿病患者中，活性氧（ROS）的大量产生、致病微生物的侵袭和高血糖水平会延迟正常的愈合过程。成功治疗糖尿病伤口需要有效的策略来控制氧化应激、促进血管生成、再上皮化和胶原沉积。在这项研究中，通过丝素（SF）的降血脂特性、黑色素的抗氧化特性和小檗碱的治疗效果的融合，开发了一种用于糖尿病状况下快速伤口愈合的复合水凝胶。研究表明，水凝胶基质的交联介孔形态有利于小檗碱的缓慢释放，从而在伤口部位赋予长期的治疗效果。该复合水凝胶配方具有生物相容性，可在体外条件下刺激成纤维细胞的有效迁移，并控制氧化应激。该水凝胶可用作组织再上皮化的支架，并促进糖尿病 I 型 Wistar 大鼠模型的伤口修复。本研究证明了载小檗碱 SF-黑色素复合水凝胶作为糖尿病伤口愈合的潜在敷料配方的能力。
