Faculdade de Ciências Farmacêuticas Alimentos e Nutrição (FACFAN), Universidade Federal de Mato Grosso Do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, Brazil.
Microb Pathog. 2023 Nov;184:106339. doi: 10.1016/j.micpath.2023.106339. Epub 2023 Sep 9.
Coronavirus disease (COVID-19) is an acute respiratory disease caused by the new coronavirus (SARS-CoV-2) that has spread throughout the world causing millions of deaths. COVID-19 promotes excessive release of pro-inflammatory cytokines leading to acute lung injury and death. Reactive oxygen species (ROS) and oxidative stress (OS) may also play a role in the pathophysiology of COVID-19. The present study investigated levels of inflammatory cytokines (IL-1β, IL-6, IL-8, IL-10, IL-12) and OS biomarkers (MPO, SOD, CAT, GST enzymes and contents of GSH, TBARS and PC) in patients with SARS-CoV-2 infection, which were correlated with disease severity. Patients with SARS significantly increased IL-1β levels, while IL-6 levels were elevated in both groups of SARS-CoV-2 positive patients. The most severe patients showed increased levels of IL-8 and IL-10, while subjects without SARS showed lower values. MPO activity were higher in both groups of SARS-CoV-2 positive patients, while SOD and CAT activity were decreased in both groups. Compared to controls, GGT was elevated only in the SARS patient group, while GST values were increased in the group of positive patients in SARS-CoV-2 without SARS and were decreased in patients with SARS. GSH and UA contents decreased in SARS-CoV-2 positive subjects, whereas TBARS and PC contents increased in both groups of SARS-CoV-2 positive patients, particularly in the SARS patient group. In addition, several important correlations were found between cytokines and the different OS parameters suggesting some inter-relationship in the complex antioxidant system of the patients. In general, patients with SARS-CoV-2 infection showed higher levels of OS biomarkers, and also elevated contents of IL-6 and IL-10, probably worsening the damage caused by SARS-CoV-2 infection. This damage may contribute to the severity of the disease and its complications, as well as a prognosis for SARS-CoV-2 patients.
新型冠状病毒（SARS-CoV-2）引发的冠状病毒病（COVID-19）是一种急性呼吸道疾病，已在全球范围内传播，导致数百万人死亡。COVID-19 会促进促炎细胞因子的过度释放，导致急性肺损伤和死亡。活性氧（ROS）和氧化应激（OS）也可能在 COVID-19 的病理生理学中发挥作用。本研究调查了 SARS-CoV-2 感染患者的炎症细胞因子（IL-1β、IL-6、IL-8、IL-10、IL-12）和 OS 生物标志物（MPO、SOD、CAT、GST 酶和 GSH、TBARS 和 PC 的含量）的水平，这些水平与疾病严重程度相关。SARS 患者的 IL-1β 水平显著升高，而两组 SARS-CoV-2 阳性患者的 IL-6 水平均升高。病情最严重的患者 IL-8 和 IL-10 水平升高，而无 SARS 的患者 IL-8 和 IL-10 水平较低。两组 SARS-CoV-2 阳性患者的 MPO 活性均升高，而两组患者的 SOD 和 CAT 活性均降低。与对照组相比，仅 SARS 患者组的 GGT 升高，而 SARS-CoV-2 无 SARS 阳性患者组的 GST 值升高，SARS 患者组的 GST 值降低。GSH 和 UA 含量在 SARS-CoV-2 阳性患者中降低，而 TBARS 和 PC 含量在两组 SARS-CoV-2 阳性患者中升高，尤其是在 SARS 患者组中升高。此外，细胞因子与不同 OS 参数之间存在一些重要相关性，这表明患者复杂抗氧化系统之间存在一些相互关系。一般来说，SARS-CoV-2 感染患者的 OS 生物标志物水平较高，IL-6 和 IL-10 含量也较高，可能会使 SARS-CoV-2 感染造成的损伤恶化。这种损伤可能导致疾病及其并发症的严重程度，以及 SARS-CoV-2 患者的预后。
