Mohammed Yasser Hussein Issa, Alghamdi Saad, Jabbar Basit, Marghani Dina, Beigh Saba, Abouzied Amr S, Khalifa Nasrin E, Khojali Weam M A, Huwaimel Bader, Alkhalifah Dalal Hussien M, Hozzein Wael N
Department of Biochemistry, Faculty of Applied Science, University of Hajjah, Hajjah, Yemen.
Department of Pharmacy, Faculty of Medicine and Medical Science, University of Al-Razi, Al-Razi, Yemen.
ACS Omega. 2023 Jul 24;8(35):32027-32042. doi: 10.1021/acsomega.3c03908. eCollection 2023 Sep 5.
Excessive use of antimicrobial medications including antibiotics has led to the emerging menace of antimicrobial resistance, which, as per the World Health Organization (WHO), is among the top ten public health threats facing humanity, globally. This necessitates that innovative technologies be sought that can aid in the elimination of pathogens and hamper the spread of infections. Zinc oxide (ZnO) has multifunctionality owing to its extraordinary physico-chemical properties and functionality in a range of applications. In this research, ZnO nanoparticles (NPs) were synthesized from zinc nitrate hexahydrate, by a green synthesis approach using extract followed by characterization of the NPs. The obtained X-ray diffraction peaks of ZnO NPs matched with the standard JCPDS card (no. 89-510). The particles had a size of 20-24 nm, a wurtzite structure with a high crystallinity, and hexagonal rod-like shape. UV-Vis spectroscopy revealed absorption peaks between 369 and 374 nm of ZnO NPs synthesized from extract confirming the formation of ZnO. Fourier transform infrared confirmed the ZnO NPs as strong absorption bands were observed in the range of 381-403 cm corresponding to Zn-O bond stretching. Negative values of the highest occupied molecular orbital-lowest unoccupied molecular orbital for ZnO NPs indicated the good potential to form a stable ligand-protein complex. Docking results indicated favorable binding interaction between ZnO and DNA gyrase subunit b with a binding energy of -2.93 kcal/mol. ZnO NPs at various concentrations inhibited the growth of and . Minimum inhibitory concentration values of ZnO NPs against and were found to be 92.07 ± 0.13 and 88.13 ± 0.35 μg/mL, respectively, at a concentration of 2 mg/mL. AO/EB staining and fluorescence microscopy revealed the ability of ZnO NPs to kill and cells. Through the findings of this study, it has been shown that extract can be used in a green synthesis approach to generate ZnO NPs, which can be employed as alternatives to antibiotics and a tool to eliminate drug-resistant microbes in the future.
包括抗生素在内的抗菌药物的过度使用导致了抗菌耐药性这一日益严重的威胁，根据世界卫生组织（WHO）的说法，这是全球人类面临的十大公共卫生威胁之一。这就需要寻找能够帮助消除病原体并阻碍感染传播的创新技术。氧化锌（ZnO）因其非凡的物理化学性质和在一系列应用中的功能而具有多功能性。在本研究中，通过使用提取物的绿色合成方法，由六水合硝酸锌合成了ZnO纳米颗粒（NPs），随后对这些NPs进行了表征。所获得的ZnO NPs的X射线衍射峰与标准JCPDS卡片（编号89-510）相匹配。颗粒尺寸为20-24nm，具有纤锌矿结构，结晶度高，呈六方棒状。紫外可见光谱显示，由提取物合成的ZnO NPs在369至374nm之间有吸收峰，证实了ZnO的形成。傅里叶变换红外光谱证实了ZnO NPs，因为在381-403cm范围内观察到对应于Zn-O键拉伸的强吸收带。ZnO NPs的最高占据分子轨道-最低未占据分子轨道的负值表明形成稳定配体-蛋白质复合物的良好潜力。对接结果表明ZnO与DNA促旋酶亚基b之间具有良好的结合相互作用，结合能为-2.93kcal/mol。不同浓度的ZnO NPs抑制了[具体菌种1]和[具体菌种2]的生长。在浓度为2mg/mL时，ZnO NPs对[具体菌种1]和[具体菌种2]的最低抑菌浓度值分别为92.07±0.13和88.13±0.35μg/mL。AO/EB染色和荧光显微镜显示ZnO NPs具有杀死[具体菌种1]和[具体菌种2]细胞的能力。通过本研究的结果表明，提取物可用于绿色合成方法来生成ZnO NPs，未来可将其用作抗生素的替代品以及消除耐药微生物的工具。
