Chen Shuai, Song Peng, Wang Yu, Wang Zeng, Xue Jiaming, Jiang Yicheng, Zhou Yan, Zhao Jie, Tang Liming
Department of Gastrointestinal Surgery, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou City, Jiangsu Province, China.
iScience. 2023 Aug 28;26(10):107756. doi: 10.1016/j.isci.2023.107756. eCollection 2023 Oct 20.
Circular RNA (circRNA) is a special category of non-coding RNA that has garnered increasing attention in the exploration of lipid metabolism. However, the functional regulation mechanisms of circRNAs in obesity diseases remain unclear. By whole transcriptome sequencing, a total of 164 circular RNAs were found to exhibit differential expression between lean and obese individuals. RT-qPCR was used to detect significant expression of circMAPK9 in obese individuals, and it was closely related to BMI. Western blot, triglyceride detection, and Oil Red O staining were employed to investigate the role of circMAPK9/hsa-miR-1322/FTO in adipogenesis. In adipocytes, the connection between hsa-miR-1322 and circMAPK9 was verified using fluorescence hybridization, luciferase reporter assay, and RNA immunoprecipitation. It was found that circMAPK9 competed for binding hsa-miR-1322 in the cytoplasm, weakening the inhibitory effect on FTO and promoting adipogenesis. Our study revealed the regulatory mechanism and important role of circMAPK9 in the process of adipogenesis.
环状RNA（circRNA）是一类特殊的非编码RNA，在脂质代谢研究中受到越来越多的关注。然而，circRNA在肥胖疾病中的功能调控机制仍不清楚。通过全转录组测序，共发现164种环状RNA在瘦人和肥胖个体之间存在差异表达。采用RT-qPCR检测肥胖个体中circMAPK9的显著表达，且其与BMI密切相关。运用蛋白质免疫印迹法、甘油三酯检测和油红O染色来研究circMAPK9/hsa-miR-1322/FTO在脂肪生成中的作用。在脂肪细胞中，利用荧光杂交、荧光素酶报告基因检测和RNA免疫沉淀验证了hsa-miR-1322与circMAPK9之间的联系。研究发现，circMAPK9在细胞质中竞争结合hsa-miR-1322，减弱了对FTO的抑制作用，促进了脂肪生成。我们的研究揭示了circMAPK9在脂肪生成过程中的调控机制和重要作用。
