You Jia, Chen Jingsheng, Hu Yanfeng, Wang Siru, Wang Jianli, Sun Tao, Shen Zhongbao
Institute of Pratacultural Science, Heilongjiang Academy of Agricultural Science, Harbin, Heilongjiang, China.
Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin, Heilongjiang, China.
Front Plant Sci. 2023 Aug 25;14:1219702. doi: 10.3389/fpls.2023.1219702. eCollection 2023.
The cytochrome P450 (CYP) genes of nematode play a crucial role in the metabolic detoxification of xenobiotics including pesticides. , also known as the soybean cyst nematode, is a sedentary endoparasite that infests plant roots, causing high annual economic losses in soybean production regions globally. In this study, we identified 36 CYP genes at a genome-wide level of the isolate TN10 using all CYPs from as queries. Subsequently, a full-length cDNA of which was significantly up-regulated by the conventional nematicide abamectin was initially cloned from . It presented significantly higher expressions in the second-stage juvenile (J2) compared to other parasitic stages of . qRT-PCR analysis suggested that the expression of was also xenobiotically induced by soybean root exudate and the metabolites of biocontrol agents. Using RNA interference (RNAi), we investigated the function of in e parasitism and nematicide selectivity. Compared to the control and -treated group, silencing of did not affect the J2 behaviors and the early invasion ability, while it decreased the number of J4s in soybean roots after 18-d inoculation with the -treated nematodes. In addition, knockdown of in resulted in an increase in J2 mortality after 24-h incubation with abamectin compared to the dsRNA-soaked and the control group. These findings revealed the potential role of in the xenobiotic detoxification pathway of . Moreover, our data also provided valuable gene information for studying the functions of the CYP family in host adaption.
线虫的细胞色素P450（CYP）基因在包括农药在内的外源化合物的代谢解毒中起着关键作用。大豆胞囊线虫，也被称为大豆胞囊线虫，是一种定居性内寄生线虫，侵染植物根系，在全球大豆产区造成每年高额的经济损失。在本研究中，我们以南方根结线虫的所有细胞色素P450为查询序列，在全基因组水平上鉴定了大豆胞囊线虫分离株TN10的36个CYP基因。随后，从大豆胞囊线虫中首次克隆了一个被传统杀线虫剂阿维菌素显著上调的全长cDNA。与大豆胞囊线虫的其他寄生阶段相比，它在二龄幼虫（J2）中的表达显著更高。qRT-PCR分析表明，大豆胞囊线虫的该基因表达也受到大豆根分泌物和生物防治剂代谢产物的外源诱导。利用RNA干扰（RNAi）技术，我们研究了该基因在大豆胞囊线虫寄生和杀线虫剂选择性中的功能。与对照组和浸泡dsRNA的处理组相比，该基因沉默不影响J2行为和早期侵染能力，但在用浸泡dsRNA处理的线虫接种18天后，大豆根中J4的数量减少。此外，与浸泡dsRNA和对照组相比，在大豆胞囊线虫中敲低该基因后，用阿维菌素孵育24小时后J2死亡率增加。这些发现揭示了该基因在大豆胞囊线虫外源解毒途径中的潜在作用。此外，我们的数据也为研究CYP家族在大豆胞囊线虫宿主适应性中的功能提供了有价值的基因信息。
