School of Forestry, Northeast Forestry University, Harbin 150040, China.
Plant Science, Wageningen University & Research, 6708 PB Wageningen, The Netherlands.
Genes (Basel). 2022 Oct 27;13(11):1956. doi: 10.3390/genes13111956.
(PWN) causes pine wilt disease (PWD), which is one of the most devastating pine diseases worldwide. Cytochrome P450 (CYP) catalyzes the biosynthetic metabolism of terpenoids and plays an important role in the modification of secondary metabolites in all living organisms. We investigated the molecular characteristics and biological functions of in . The bioinformatics analysis results indicated that has a transmembrane domain and could dock with L(-)-carvone. The gene expression pattern indicated that was expressed in 0.2, 0.4, 0.6, 0.8, and 1.0 mg/mL L(-)-carvone solutions. The - expression increased in a dose-dependent manner and peaked at 24 h of exposure when the L(-)-carvone solution concentration was 0.8 mg/mL. However, the gene expression peaked at 0.6 mg/mL after 36 h. Furthermore, RNA interference (RNAi) indicated that played an essential role in the response to L(-)-carvone. The mortality rates of the knockdown groups were higher than those of the control groups in the 0.4, 0.6, 0.8, and 1.0 mg/mL carvone solutions after 24 h of exposure or 36 h of exposure. In summary, bioinformatics provided the structural characteristics and conserved sequence properties of and its encoded protein, which provided a target gene for the study of the P450 family of . Gene silencing experiments clarified the function of in the immune defense of This study provides a basis for the screening of new molecular targets for the prevention and management of .
(PWN) 引起松材线虫病 (PWD)，这是世界上最具破坏性的松树疾病之一。细胞色素 P450 (CYP) 催化萜类化合物的生物合成代谢，在所有生物体中次生代谢物的修饰中发挥重要作用。我们研究了 在 中的分子特征和生物学功能。生物信息学分析结果表明 具有跨膜结构域，可以与 L(-)-香芹酮结合。基因表达模式表明 ，在 0.2、0.4、0.6、0.8 和 1.0 mg/mL L(-)-香芹酮溶液中表达。- 表达呈剂量依赖性增加，当 L(-)-香芹酮溶液浓度为 0.8 mg/mL 时，暴露 24 小时达到峰值。然而，在 36 小时后，基因表达达到峰值。此外，RNA 干扰 (RNAi) 表明 在对 L(-)-香芹酮的反应中起着重要作用。在暴露 24 小时或 36 小时后，在 0.4、0.6、0.8 和 1.0 mg/mL 香芹酮溶液中， 敲低组的死亡率均高于对照组。总之，生物信息学提供了 及其编码蛋白的结构特征和保守序列特性，为 P450 家族的研究提供了一个靶基因。基因沉默实验阐明了 在 免疫防御中的功能。本研究为筛选防治 的新分子靶标提供了依据。
