Li Siyuan, Chen Diyang, Zhang Huamin, Yang Yong, Huai Jianguo, Huang Linna, Fan Kai, Lin Tongyuan, Ding Baijing
Postgraduate School, Wannan Medical College Wuhu 241000, Anhui, China.
Department of Gastroenterology, The First People's Hospital of Wuhu Wuhu 241000, Anhui, China.
Int J Clin Exp Pathol. 2023 Aug 15;16(8):199-208. eCollection 2023.
As a tumor suppressor gene, zinc finger protein 471 (ZNF471) has an essential role in tumor occurrence and development. Due to promoter hypermethylation, it can be underexpressed or silenced in gastric cancer (GC) cell lines. In this study, we investigated relationships between clinical characteristics and ZNF471 expression levels in tissues of patients with GC.
We used immunohistochemistry (IHC) to detect ZNF471 expression in paraffin tissue specimens, and quantitative real-time PCR (qRT-PCR) and western blot (WB) analysis to measure expression levels of ZNF471 in fresh tissue specimens. We analyzed relationships between ZNF471 expression levels and characteristics, such as tumor size, gender, age, TNM stage, and lymph node metastasis.
Immunohistochemistry revealed the expression of ZNF471 protein from paraffin blocks of GC tissues was significantly lower than that of adjacent tissues. Expression levels of ZNF471 mRNA and protein in fresh GC tissues were markedly lower than those in adjacent tissues and in normal gastric mucosal tissues from healthy subjects. ZNF471 expression was significantly correlated with tumor size, lymph node metastasis, and TNM stage (all <0.05). There were no significant associations with gender, age, distant metastasis, or pathologic type. Expression of ZNF471 mRNA and protein was not significantly different between adjacent tissues of patients with GC and normal gastric mucosal tissue from healthy subjects.
ZNF471 functions as a tumor suppressor during the pathogenesis of GC. Thus, it is a promising biomarker for diagnosis and therapy of GC.
作为一种肿瘤抑制基因，锌指蛋白471（ZNF471）在肿瘤的发生发展中起重要作用。由于启动子高甲基化，它在胃癌（GC）细胞系中可能表达不足或沉默。在本研究中，我们调查了GC患者组织中临床特征与ZNF471表达水平之间的关系。
我们采用免疫组织化学（IHC）检测石蜡组织标本中ZNF471的表达，并用定量实时聚合酶链反应（qRT-PCR）和蛋白质免疫印迹（WB）分析来测量新鲜组织标本中ZNF471的表达水平。我们分析了ZNF471表达水平与肿瘤大小、性别、年龄、TNM分期和淋巴结转移等特征之间的关系。
免疫组织化学显示，GC组织石蜡块中ZNF471蛋白的表达明显低于相邻组织。新鲜GC组织中ZNF471 mRNA和蛋白的表达水平明显低于相邻组织以及健康受试者的正常胃黏膜组织。ZNF471表达与肿瘤大小、淋巴结转移和TNM分期显著相关（均P<0.05）。与性别、年龄、远处转移或病理类型无显著关联。GC患者相邻组织与健康受试者正常胃黏膜组织之间ZNF471 mRNA和蛋白的表达无显著差异。
ZNF471在GC发病机制中起肿瘤抑制作用。因此，它是GC诊断和治疗中有前景的生物标志物。
