Department of Gastroenterology, The First People's Hospital of Yichang, Yichang, Hubei, China.
Department of Gastroenterology, The People's Hospital of Three Gorges University, Yichang, Hubei, China.
Technol Cancer Res Treat. 2019 Jan 1;18:1533033819874783. doi: 10.1177/1533033819874783.
This study aims to investigate the expression, role, and detailed mechanism of microRNA-204-5p and zinc finger protein 521 in gastric cancer.
Immunohistochemistry was adopted to detect the expressions of zinc finger protein 521 in 82 cases of gastric cancer tissues. Western blot was used to detect the expressions of zinc finger protein 521 in gastric cancer cells and adjacent cells. Moreover, the correlation between zinc finger protein 521 and the prognosis of patients were also evaluated. Cell Counting Kit 8 assay and colony formation assay were performed to figure out the impact of zinc finger protein 521 on the proliferation of gastric cancer cells. By conducting flow cytometry, the effect of zinc finger protein 521 on the apoptosis of gastric cancer cells was determined. The scratch wound healing assay and transwell invasion assay were carried out to determine the effect of zinc finger protein 521 on regulating the motility and invasion of gastric cancer cells. Ultimately, the targeting relationship and interaction between microRNA-204-5p and zinc finger protein 521 were verified by real-time polymerase chain reaction, Western blot, and dual luciferase reporter gene assay.
Compared with adjacent cells, zinc finger protein 521 was highly expressed in gastric cancer cells, which was related to TNM stage ( = .0388), tumor size ( = .0168), and local lymph node metastasis ( = .0024). Overexpressed zinc finger protein 521 can promote the proliferation, migration, and invasion of gastric cancer cells and inhibit the apoptosis. Zinc finger protein 521 is a target gene of microRNA-106-5p, and there was a negative correlation between the expression of zinc finger protein 521 and microRNA-204-5p.
Zinc finger protein 521 can arrest the apoptosis and enhance the proliferation, migration, and invasion of gastric cancer cells via regulating microRNA-204-5p. Our study may provide novel clues for the treatment of patients with gastric cancer.
本研究旨在探讨 microRNA-204-5p 和锌指蛋白 521 在胃癌中的表达、作用及详细机制。
采用免疫组织化学法检测 82 例胃癌组织中锌指蛋白 521 的表达，Western blot 法检测胃癌细胞及相邻细胞中锌指蛋白 521 的表达，评估锌指蛋白 521 与患者预后的相关性。通过细胞计数试剂盒 8 法和集落形成实验检测锌指蛋白 521 对胃癌细胞增殖的影响。通过流式细胞术检测锌指蛋白 521 对胃癌细胞凋亡的影响。划痕愈合实验和 Transwell 侵袭实验检测锌指蛋白 521 对胃癌细胞运动和侵袭的调节作用。最后，通过实时聚合酶链反应、Western blot 和双荧光素酶报告基因实验验证 microRNA-204-5p 和锌指蛋白 521 之间的靶向关系和相互作用。
与相邻细胞相比，锌指蛋白 521 在胃癌细胞中高表达，与 TNM 分期（ =.0388）、肿瘤大小（ =.0168）和局部淋巴结转移（ =.0024）有关。过表达锌指蛋白 521 可促进胃癌细胞的增殖、迁移和侵袭，抑制凋亡。锌指蛋白 521 是 microRNA-106-5p 的靶基因，锌指蛋白 521 的表达与 microRNA-204-5p 呈负相关。
锌指蛋白 521 可通过调节 microRNA-204-5p 抑制胃癌细胞凋亡，增强其增殖、迁移和侵袭能力。本研究可能为胃癌患者的治疗提供新的线索。
