El-Guourrami Otman, Elbouny Hamza, Ait Benlabchir Abdessamad, Drioua Soufiane, Ouahzizi Brahim, Alem Chakib, Doukkali Anass, Benzeid Hanane
Laboratory of Analytical Chemistry, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco.
Laboratory of Biochemistry, Department of Biology, Faculty of Sciences and Techniques, University Moulay Ismail, Errachidia, Morocco.
J Taibah Univ Med Sci. 2023 Jul 31;18(6):1557-1566. doi: 10.1016/j.jtumed.2023.07.011. eCollection 2023 Dec.
OBJECTIVE: The main purpose of the present work was to determine the chemical composition, safety, and antioxidant and antihyperlipidemic activities of an aqueous extract of . METHODS: Phytochemical analysis (total phenolic, total flavonoid, and total hydroxycinnamic acid contents), antioxidant activity (ferric-reducing antioxidant power, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid, 2,2-diphenyl-1-picrylhydrazil, and total antioxidant capacity tests), acute toxicity, and antihyperlipidemic activity were evaluated according to established models. In addition, the phytochemical profile was determined by methylation followed by gas chromatography-mass spectrometry (GC/MS). RESULTS: The aqueous extract of had a high content of total polyphenols (87.01 ± 0.31 mg gallic acid equivalent (GAE)/g extract) and hydroxycinnamic acid (2.28 ± 0.1 g/100 g Powdered Material) and a low content of total flavonoids (2.99 ± 0.16 mg GAE/g extract). In addition, the extract demonstrated remarkable antioxidant activity (DPPH IC = 76.67 ± 0.56 μg/mL, ABTS IC = 89.65 ± 0.27 μg/mL, FRAP EC = 296.32 ± 0.86 μg/mL, TAC value = 43 ± 0.27 mg EAA/g extract). The main compounds were identified as benzene, (hexyloxy)- (19.32%), 2,6a-methano-6aH-indeno[4,5-b]oxirene, octahedro-(1a.alpha., 2.beta., 3a.alpha., 6a.beta., 6b.alpha.)- (32.42%), d-fucose (5.47%), 5-hydroxymethylfurfural (5.47%) and guaiacol (3.19%). The LD was estimated to be between 500 and 2000 mg/kg. Furthermore, at 500 and 250 mg/kg, the aqueous extract of exhibited good antihyperlipidemic activity CONCLUSION: extract has significant pharmacological potential and a varied chemical composition
目的:本研究的主要目的是确定[提取物名称]水提取物的化学成分、安全性、抗氧化和抗高血脂活性。 方法:根据既定模型评估植物化学分析(总酚、总黄酮和总羟基肉桂酸含量)、抗氧化活性(铁还原抗氧化能力、2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)、2,2-二苯基-1-苦基肼自由基清除能力和总抗氧化能力测试)、急性毒性和抗高血脂活性。此外,通过甲基化后气相色谱-质谱联用(GC/MS)确定植物化学图谱。 结果:[提取物名称]水提取物总多酚含量高(87.01±0.31毫克没食子酸当量(GAE)/克提取物),羟基肉桂酸含量高(2.28±0.1克/100克粉末材料),总黄酮含量低(2.99±0.16毫克GAE/克提取物)。此外,该提取物表现出显著的抗氧化活性(DPPH IC₅₀ = 76.67±0.56微克/毫升,ABTS IC₅₀ = 89.65±0.27微克/毫升,FRAP EC₅₀ = 296.32±0.86微克/毫升,TAC值 = 43±0.27毫克EAA/克提取物)。主要化合物鉴定为苯,(己氧基)-(19.32%),2,6a-亚甲基-6aH-茚并[4,5-b]环氧乙烷,八面体-(1a.α., 2.β., 3a.α., 6a.β., 6b.α.)-(32.42%),D-岩藻糖(5.47%),5-羟甲基糠醛(5.47%)和愈创木酚(3.19%)。LD₅₀估计在500至2000毫克/千克之间。此外,在500和250毫克/千克时,[提取物名称]水提取物表现出良好的抗高血脂活性。 结论:[提取物名称]提取物具有显著的药理潜力和多样的化学成分 。
需注意,原文中部分提取物名称未给出具体内容,翻译时用[提取物名称]代替。
J Taibah Univ Med Sci. 2023-7-31
Pharmacognosy Res. 2016
Int J Environ Health Res. 2018-12-20
Pharmacognosy Res. 2016
Evid Based Complement Alternat Med. 2020-11-12
Mutat Res Genet Toxicol Environ Mutagen. 2020-4
Evid Based Complement Alternat Med. 2019-7-21
Int J Prev Med. 2018-3-9