El-Guourrami Otman, Elbouny Hamza, Ait Benlabchir Abdessamad, Drioua Soufiane, Ouahzizi Brahim, Alem Chakib, Doukkali Anass, Benzeid Hanane
Laboratory of Analytical Chemistry, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco.
Laboratory of Biochemistry, Department of Biology, Faculty of Sciences and Techniques, University Moulay Ismail, Errachidia, Morocco.
J Taibah Univ Med Sci. 2023 Jul 31;18(6):1557-1566. doi: 10.1016/j.jtumed.2023.07.011. eCollection 2023 Dec.
The main purpose of the present work was to determine the chemical composition, safety, and antioxidant and antihyperlipidemic activities of an aqueous extract of .
Phytochemical analysis (total phenolic, total flavonoid, and total hydroxycinnamic acid contents), antioxidant activity (ferric-reducing antioxidant power, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid, 2,2-diphenyl-1-picrylhydrazil, and total antioxidant capacity tests), acute toxicity, and antihyperlipidemic activity were evaluated according to established models. In addition, the phytochemical profile was determined by methylation followed by gas chromatography-mass spectrometry (GC/MS).
The aqueous extract of had a high content of total polyphenols (87.01 ± 0.31 mg gallic acid equivalent (GAE)/g extract) and hydroxycinnamic acid (2.28 ± 0.1 g/100 g Powdered Material) and a low content of total flavonoids (2.99 ± 0.16 mg GAE/g extract). In addition, the extract demonstrated remarkable antioxidant activity (DPPH IC = 76.67 ± 0.56 μg/mL, ABTS IC = 89.65 ± 0.27 μg/mL, FRAP EC = 296.32 ± 0.86 μg/mL, TAC value = 43 ± 0.27 mg EAA/g extract). The main compounds were identified as benzene, (hexyloxy)- (19.32%), 2,6a-methano-6aH-indeno[4,5-b]oxirene, octahedro-(1a.alpha., 2.beta., 3a.alpha., 6a.beta., 6b.alpha.)- (32.42%), d-fucose (5.47%), 5-hydroxymethylfurfural (5.47%) and guaiacol (3.19%). The LD was estimated to be between 500 and 2000 mg/kg. Furthermore, at 500 and 250 mg/kg, the aqueous extract of exhibited good antihyperlipidemic activity
extract has significant pharmacological potential and a varied chemical composition
本研究的主要目的是确定[提取物名称]水提取物的化学成分、安全性、抗氧化和抗高血脂活性。
根据既定模型评估植物化学分析（总酚、总黄酮和总羟基肉桂酸含量）、抗氧化活性（铁还原抗氧化能力、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）、2,2-二苯基-1-苦基肼自由基清除能力和总抗氧化能力测试）、急性毒性和抗高血脂活性。此外，通过甲基化后气相色谱-质谱联用（GC/MS）确定植物化学图谱。
[提取物名称]水提取物总多酚含量高（87.01±0.31毫克没食子酸当量（GAE）/克提取物），羟基肉桂酸含量高（2.28±0.1克/100克粉末材料），总黄酮含量低（2.99±0.16毫克GAE/克提取物）。此外，该提取物表现出显著的抗氧化活性（DPPH IC₅₀ = 76.67±0.56微克/毫升，ABTS IC₅₀ = 89.65±0.27微克/毫升，FRAP EC₅₀ = 296.32±0.86微克/毫升，TAC值 = 43±0.27毫克EAA/克提取物）。主要化合物鉴定为苯，（己氧基）-（19.32%），2,6a-亚甲基-6aH-茚并[4,5-b]环氧乙烷，八面体-（1a.α., 2.β., 3a.α., 6a.β., 6b.α.）-（32.42%），D-岩藻糖（5.47%），5-羟甲基糠醛（5.47%）和愈创木酚（3.19%）。LD₅₀估计在500至2000毫克/千克之间。此外，在500和250毫克/千克时，[提取物名称]水提取物表现出良好的抗高血脂活性。
[提取物名称]提取物具有显著的药理潜力和多样的化学成分 。
需注意，原文中部分提取物名称未给出具体内容，翻译时用[提取物名称]代替。
