Zafar Adil, Tripathi Vartika, Khan Mariyam, Manglam Keshav K, Rastogi Preeti, Almotreb Abdullah M
Private Practitioner, Conservative Dentistry and Endodontics, Balia, Uttar Pradesh, India.
Department of Orthodontics, Ahmedabad Dental College and Hospital, Ahmedabad, Gujarat, India.
J Pharm Bioallied Sci. 2023 Jul;15(Suppl 2):S892-S894. doi: 10.4103/jpbs.jpbs_72_23. Epub 2023 Jul 11.
To evaluate the antimicrobial activity of PRP and PRF with and without nanosilver.
The materials were tested in powdered form is nanosilver. The nanosilver particles was mixed to form with PRP and PRF so as to placed in a wells followed the groups are experimental groups; Group I: PRP + nanosilver particles, Group II: PRF + nanosilver and control group: PRP and PRF and normal saline. Silver nanoparticles was tested at concentrations of 50 μ gram per mL. The powder was prepared for each group with identical amount of the powder (milligram/mg) and then mixed with 1 milliliter liquid. The plates are then incubated at 37°C under appropriate atmospheric conditions (80% N, 10% CO 10% H) for 24 hours, 48 hours, and 72 hours under anaerobic conditions in a CO incubator. The diameters of the zones of bacterial and fungal growth inhibition around the wells containing the test substances are then recorded after the period of incubation. The inhibitory zone determined in millimeter by measuring scale the shortest distance between the outer margin of the well and initial microbial as well as fungal growth. The experiments were performed 20 times and the mean and standard deviations of the inhibitory zones were calculated.
Platelet rich fibrin is mixed with nanosilver particles showed higher antimicrobial efficacy than platelet rich plasma with nanosilver and simple platelet rich plasma and platelet rich fibrin are equivalent when it is placed against the anaerobic bacteria E.faecalis and yeast like fungi Candida albicans, respectively.
Groups presented with antimicrobial efficacy in this order- Group IV > Group II > Group III > Group I.
评估含和不含纳米银的富血小板血浆（PRP）和富血小板纤维蛋白（PRF）的抗菌活性。
材料以纳米银粉末形式进行测试。将纳米银颗粒与PRP和PRF混合，然后放入孔中，分组如下：实验组；第一组：PRP + 纳米银颗粒，第二组：PRF + 纳米银，对照组：PRP、PRF和生理盐水。纳米银颗粒的测试浓度为每毫升50微克。为每组准备相同量（毫克/mg）的粉末，然后与1毫升液体混合。然后将平板在37°C下，在适当的大气条件（80% N、10% CO₂、10% H₂）下，于二氧化碳培养箱中厌氧培养24小时、48小时和72小时。培养期结束后，记录含测试物质的孔周围细菌和真菌生长抑制圈的直径。通过测量尺度确定抑制圈的直径，即孔的外缘与初始微生物以及真菌生长之间的最短距离，以毫米为单位。实验进行20次，并计算抑制圈的平均值和标准差。
与纳米银颗粒混合的富血小板纤维蛋白对粪肠球菌和白色念珠菌等厌氧菌的抗菌效果高于含纳米银的富血小板血浆，而单纯的富血小板血浆和富血小板纤维蛋白在抗菌效果上相当。
各实验组抗菌效果顺序为：第四组 > 第二组 > 第三组 > 第一组。
