Key Laboratory of Integrated Management of Crop Disease and Pests, Ministry of Education, 210095 Nanjing, China.
Key Laboratory of Soybean Disease and Pest Control of Ministry of Agriculture and Rural Affairs, 210095 Nanjing, China.
J Agric Food Chem. 2023 Sep 20;71(37):13795-13804. doi: 10.1021/acs.jafc.3c03265. Epub 2023 Sep 11.
Moths rely on plant volatiles to locate appropriate plants for feeding and laying eggs. While extensive research has been conducted on the global agricultural pests, and , their molecular mechanisms for detecting plant volatiles remain mostly unknown. Here, we have demonstrated that nonanal, a common plant volatile, is attractive for both virgin and gravid females of the two species. Second, we have identified a conserved odorant receptor clade (SfruOR47 clade) that is primarily tuned to nonanal. Finally, by three-dimensional (3D) structure prediction, molecular docking, and site-directed mutagenesis, we have revealed that the His57 and Glu61 residues, also shared by other six orthologous ORs, are essential for nonanal binding in SfruOR47 and SlituOR9, indicating the conserved structure and function of ORs in the SfruOR47 clade. These findings offer novel insights into the molecular mechanisms and evolutionary aspects of moth behavior in response to plant volatiles.
蛾类依靠植物挥发物来定位适宜的取食和产卵植物。尽管对全球农业害虫 和 进行了广泛的研究，但它们检测植物挥发物的分子机制在很大程度上仍不清楚。在这里，我们证明了壬醛，一种常见的植物挥发物，对这两个物种的处女和怀孕雌虫都具有吸引力。其次，我们鉴定了一个保守的气味受体进化枝（SfruOR47 进化枝），它主要对壬醛敏感。最后，通过三维（3D）结构预测、分子对接和定点突变，我们揭示了 His57 和 Glu61 残基，也被其他六个同源 OR 所共有，对 SfruOR47 和 SlituOR9 中的壬醛结合是必需的，表明 OR 在 SfruOR47 进化枝中的保守结构和功能。这些发现为昆虫对植物挥发物的行为的分子机制和进化方面提供了新的见解。
