Wang Zhiqiang, Wang Xiaoqing, Liu Weihao, Chen Run, Liu Yang
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.
Insects. 2024 Jul 26;15(8):564. doi: 10.3390/insects15080564.
In the past decade, has emerged as a significant invasive pest globally, posing a serious threat to agriculture due to its broad diet, migratory behavior, and ability to cause extensive plant damage. While extensive research has focused on the olfactory capabilities of adult , understanding of the olfactory process in larvae remains limited, despite larvae playing a crucial role in crop damage. To address this gap, we identified an odorant receptor (OR), SfruOR40, expressed in the first-instar larvae through phylogenetic analysis. Using quantitative real-time PCR, we compared SfruOR40 expression levels in larvae and adults. We then characterized the function of SfruOR40 against 67 compounds using the oocyte expression system and found that SfruOR40 responded to three plant volatiles. Further, behavioral experiments revealed a larval attraction to (-)--Caryophyllene oxide. This study elucidates SfruOR40's role in the olfactory recognition of newly hatched larvae, expanding our knowledge of such mechanisms in Noctuid moths. Furthermore, it highlights the potential of plant-derived natural products for biological pest control from a behavioral ecology perspective.
在过去十年中，[某种昆虫名称]已成为全球一种重要的入侵性害虫，由于其食性广泛、具有迁飞行为且能够对植物造成广泛损害，因而对农业构成严重威胁。尽管大量研究聚焦于成虫的嗅觉能力，但尽管幼虫在作物损害中起着关键作用，对其嗅觉过程的了解仍然有限。为填补这一空白，我们通过系统发育分析鉴定出一种在一龄幼虫中表达的气味受体（OR），即SfruOR40。我们使用定量实时PCR比较了幼虫和成虫中SfruOR40的表达水平。然后，我们利用卵母细胞表达系统针对67种化合物对SfruOR40的功能进行了表征，发现SfruOR40对三种植物挥发物有反应。此外，行为实验揭示了幼虫对（-）-氧化石竹烯有吸引力。本研究阐明了SfruOR40在新孵化的[某种昆虫名称]幼虫嗅觉识别中的作用，扩展了我们对夜蛾科昆虫此类机制的认识。此外，它从行为生态学角度突出了植物源天然产物在生物防治害虫方面的潜力。
