Nady Ouais Ghada, Hassan Doaa Mohamad
Department of Human Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Department of Human Anatomy and Embryology, Faculty of Medicine, Newgiza University, Giza, Egypt.
Anat Cell Biol. 2023 Dec 31;56(4):538-551. doi: 10.5115/acb.23.101. Epub 2023 Sep 12.
Exposure to environmental pollutants such as carbon tetrachloride (CCL) causes liver damage. This study aimed to compare the ameliorative activity of the dates flesh extract (DFE) and selenium-nanoparticles (SeNPs) on CCL-induced hepatotoxicity and if DFE could be a useful alternative supplement. Twenty-four male albino rats were enrolled and randomly divided into four equal groups (6 rats in each group): control group received only basal diet with no medications. Group II received CCL in a dose of 0.5 mg/kg intraperitoneal injection twice weekly for four weeks. Group III rats were pretreated with SeNPs in a dose of 2.5 mg/kg once a day orally three times/wk for four weeks alone then combined with the previously described dose of CCL for another four weeks. Group IV rats were pretreated with DFE in a dose of 8 ml of the aqueous extract/kg/d orally for four weeks alone then combined with the previously described dose of CCL for another four weeks. The liver damage was assessed by estimation of plasma concentration of albumin and enzymes activities of alanine aminotransferase and tissue genes expression. Liver oxidation levels were assessed by measuring the tissue concentration of the malondialdehyde, superoxide dismutase, and the total glutathione. Additionally, inflammatory mediators tumour necrosis factor--α and interleukin-6 were estimated. Detecting the liver's cellular structural damage was done by histopathological and immunohistochemical examination. This study suggests that CCL-induced liver damage in rats can be protected by administration whether the costly SeNPs or the economical DFE.
接触四氯化碳（CCL）等环境污染物会导致肝脏损伤。本研究旨在比较枣果肉提取物（DFE）和硒纳米颗粒（SeNPs）对CCL诱导的肝毒性的改善活性，以及DFE是否可以作为一种有用的替代补充剂。招募了24只雄性白化大鼠，并随机分为四组，每组6只：对照组仅接受基础饮食，不服用药物。第二组每周两次腹腔注射剂量为0.5mg/kg的CCL，持续四周。第三组大鼠单独口服剂量为2.5mg/kg的SeNPs，每周三次，持续四周，然后与上述剂量的CCL联合使用，再持续四周。第四组大鼠单独口服剂量为8ml/kg/d的DFE水提取物，持续四周，然后与上述剂量的CCL联合使用，再持续四周。通过估计血浆白蛋白浓度、丙氨酸转氨酶活性和组织基因表达来评估肝损伤。通过测量丙二醛、超氧化物歧化酶和总谷胱甘肽的组织浓度来评估肝脏氧化水平。此外，还评估了炎症介质肿瘤坏死因子-α和白细胞介素-6。通过组织病理学和免疫组织化学检查来检测肝脏的细胞结构损伤。本研究表明，无论是昂贵的SeNPs还是经济的DFE给药，都可以保护大鼠免受CCL诱导的肝损伤。
