Department of Otolaryngology - Head & Neck Surgery, School of Medicine, Stanford University, Stanford, California, U.S.A.
Department of Anesthesiology, Perioperative and Pain Medicine, School of Medicine, Stanford University, Stanford, California, U.S.A.
Laryngoscope. 2024 Mar;134(3):1316-1326. doi: 10.1002/lary.31043. Epub 2023 Sep 12.
The effects of electronic cigarettes (e-cigarettes) on the larynx are relatively unknown. This study examined the short-term effects of e-cigarette inhalation on cellular and inflammatory responses within the mouse laryngeal glottic and subglottic regions after exposure to pod-based devices (JUUL).
Male C57BL6/J mice (8-9 weeks) were assigned to control (n = 9), JUUL flavors Mint (JMi; n = 10) or Mango (JMa; n = 10). JUUL mice were exposed to 2 h/day for 1, 5, and 10 days using the inExpose inhalation system. Control mice were in room air. Vocal fold (VF) epithelial thickness, cell proliferation, subglandular area and composition, inflammatory cell infiltration, and surface topography were evaluated in the harvested larynges. Mouse body weight and urinary nicotine biomarkers were also measured. Chemical analysis of JUUL aerosols was conducted using selective ion flow tube mass spectrometry.
JUUL-exposed mice had reduced body weight after day 5. Urinary nicotine biomarker levels indicated successful JUUL exposure and metabolism. Quantitative analysis of JUUL aerosol indicated that chemical constituents differ between JMi and JMa flavors. VF epithelial thickness, cellular proliferation, glandular area, and surface topography remained unchanged after JUUL exposures. Acidic mucus content increased after 1 day of JMi exposure. VF macrophage and T-cell levels slightly increased after 10 days of JMi exposures.
Short-term e-cigarette exposures cause minimal flavor- and region-specific cellular and inflammatory changes in the mouse larynx. This work provides a foundation for long-term studies to determine if these responses are altered with multiple e-cigarette components and concentrations.
N/A Laryngoscope, 134:1316-1326, 2024.
电子烟（e-cigarettes）对喉部的影响尚不清楚。本研究使用 pod 式装置（JUUL）检测了电子烟吸入对小鼠声门和下喉部细胞和炎症反应的短期影响。
将雄性 C57BL6/J 小鼠（8-9 周）分为对照组（n=9）、JUUL 薄荷味（JMi；n=10）或芒果味（JMa；n=10）。使用 inExpose 吸入系统，将 JUUL 小鼠每天暴露 2 小时，持续 1、5 和 10 天。对照组小鼠置于室内空气环境中。评估采集的喉组织中的声带（VF）上皮厚度、细胞增殖、Sub 下腺面积和成分、炎症细胞浸润和表面形貌。还测量了小鼠体重和尿液尼古丁生物标志物。使用选择性离子流管质谱法对 JUUL 气溶胶进行化学分析。
JUUL 暴露后第 5 天，小鼠体重减轻。尿液尼古丁生物标志物水平表明 JUUL 暴露和代谢成功。JUUL 气溶胶的定量分析表明，JMi 和 JMa 口味的化学成分不同。JUUL 暴露后，VF 上皮厚度、细胞增殖、腺面积和表面形貌保持不变。暴露于 JMi 1 天后，酸性黏液含量增加。暴露于 JMi 10 天后，VF 巨噬细胞和 T 细胞水平略有增加。
短期电子烟暴露导致小鼠喉部出现最小的风味和区域特异性细胞和炎症变化。这项工作为长期研究奠定了基础，以确定这些反应是否会因多种电子烟成分和浓度而改变。
无。喉镜，134:1316-1326，2024。
