Backlund P S, Carotti D, Cantoni G L
Eur J Biochem. 1986 Oct 15;160(2):245-51. doi: 10.1111/j.1432-1033.1986.tb09963.x.
The effects of 3-deazaaristeromycin and 3-deazaadenosine on RNA methylation and synthesis were examined in the mouse macrophage cell line, RAW264. S-Adenosylhomocysteine accumulated in cells incubated with 3-deazaaristeromycin while S-3-deazaadenosylhomocysteine was the major product in cells incubated with 3-deazaadenosine and homocysteine thiolactone. RNA methylation was inhibited to a similar extent by the accumulation of either S-adenosylhomocysteine or S-3-deazaadenosylhomocysteine, with S-adenosylhomocysteine being a slightly better inhibitor. In mRNA, the synthesis of N6-methyladenosine and N6-methyl-2'-O-methyladenosine were inhibited to the greatest extent, while the synthesis of 7-methylguanosine and 2'-O-methyl nucleosides were inhibited to a lesser extent. Incubation of cells with 100 microM 3-deazaaristeromycin or with 10 microM 3-deazaadenosine and 50 microM homocysteine thiolactone produced little inhibition of mRNA synthesis, even though mRNA methylation was inhibited. In contrast, mRNA synthesis was greatly inhibited by treatment of cells with 100 microM 3-deazaadenosine and the inhibition of synthesis was not correlated with an inhibition of methylation.
在小鼠巨噬细胞系RAW264中检测了3-脱氮阿糖胞苷和3-脱氮腺苷对RNA甲基化和合成的影响。在用3-脱氮阿糖胞苷孵育的细胞中积累了S-腺苷同型半胱氨酸，而在用3-脱氮腺苷和同型半胱氨酸硫内酯孵育的细胞中，S-3-脱氮腺苷同型半胱氨酸是主要产物。S-腺苷同型半胱氨酸或S-3-脱氮腺苷同型半胱氨酸的积累对RNA甲基化的抑制程度相似，S-腺苷同型半胱氨酸是稍好的抑制剂。在mRNA中，N6-甲基腺苷和N6-甲基-2'-O-甲基腺苷的合成受到的抑制最大，而7-甲基鸟苷和2'-O-甲基核苷的合成受到的抑制较小。用100μM 3-脱氮阿糖胞苷或10μM 3-脱氮腺苷和50μM同型半胱氨酸硫内酯孵育细胞，即使mRNA甲基化受到抑制，对mRNA合成的抑制也很小。相反，用100μM 3-脱氮腺苷处理细胞可极大地抑制mRNA合成，且合成的抑制与甲基化的抑制无关。
