人类免疫缺陷病毒精英控制者、人类免疫缺陷病毒感染以及与乙型肝炎病毒或丙型肝炎病毒合并感染中的新潜在 microRNA 生物标志物。

New Potential MicroRNA Biomarkers in Human Immunodeficiency Virus Elite Controllers, Human Immunodeficiency Virus Infections, and Coinfections with Hepatitis B Virus or Hepatitis C Virus.

机构信息

Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, Erbil, Iraq.

AJA University of Medical Sciences, Golestan Hospital Research Center, Tehran, Iran.

出版信息

Intervirology. 2023;66(1):122-135. doi: 10.1159/000533595. Epub 2023 Sep 12.

DOI:10.1159/000533595

PMID:37699384

Abstract

INTRODUCTION

This research aimed to evaluate the specific microRNA (miRNA) including miR-17-5p, miRN-140-3p miR-191-5p, miR-200c-3p, and miR-N367 and cellular factors (p21, SDF-1, XCL1, CCL-2, and IL-2) in controlling replication of human immunodeficiency virus (HIV) in ECs.

METHODS

The expression of miRNAs was assessed between healthy control groups and patient groups including ART-naïve HIV, HIV ART, ECs, and coinfection (HIV-HBV and HIV-HCV) via real-time PCR technique. Besides, the expression level of the nef gene and cellular factors were assessed by the ELISA method. The differences in the level of cellular factors and selected miRNAs between study groups were analyzed using the Kruskal-Wallis H or one-way ANOVA test. In addition, the potential of selected miRNAs as biomarkers for discriminating study groups was assessed by the receiver-operator characteristic (ROC) curve analysis.

RESULTS

Some miRNAs in ECs, HIV ART, and healthy controls have similar expression patterns, whereas a miRNA expression profile of patient groups significantly differed compared to EC and control groups. According to ROC curve analyses, the miR-17-5p, miR-140-3p miR-191-5p, miR-200c-3p, and miR-N367 can be served as biomarkers for discriminating ECs from ART-naïve HIV-infected groups. There was a significant correlation between some miRNAs and cellular factors/the viral load as well.

CONCLUSION

This report demonstrated a differentiation in the expression of selected immunological factors and cellular/viral miRNAs in ECs compared to other patient groups. Some miRNAs and cellular factors are involved in the viral replication control, immune response/modulation and can be used as biomarkers for diagnosis of ECs and differentiation with other groups. Differential expression of these miRNAs and cellular factors in different stages of HIV infection can help in finding novel ways for infection control.

摘要

简介

本研究旨在评估特定的 microRNA（miRNA），包括 miR-17-5p、miR-140-3p、miR-191-5p、miR-200c-3p 和 miR-N367 以及细胞因子（p21、SDF-1、XCL1、CCL-2 和 IL-2）在控制人免疫缺陷病毒（HIV）在 ECs 中的复制。

方法

通过实时 PCR 技术评估健康对照组和患者组（包括未经 ART 治疗的 HIV、ART 治疗的 HIV、ECs 和合并感染（HIV-HBV 和 HIV-HCV））之间 miRNA 的表达。此外，通过 ELISA 法评估 nef 基因和细胞因子的表达水平。使用 Kruskal-Wallis H 或单向 ANOVA 检验分析研究组之间细胞因子和选定 miRNA 水平的差异。此外，通过接收者操作特征（ROC）曲线分析评估选定 miRNA 作为区分研究组的生物标志物的潜力。

结果

ECs、HIV ART 和健康对照组中的一些 miRNA 具有相似的表达模式，而患者组的 miRNA 表达谱与 EC 和对照组显著不同。根据 ROC 曲线分析，miR-17-5p、miR-140-3p miR-191-5p、miR-200c-3p 和 miR-N367 可作为区分 EC 与未经 ART 治疗的 HIV 感染组的生物标志物。一些 miRNA 与细胞因子/病毒载量之间也存在显著相关性。

结论

本报告表明，与其他患者组相比，ECs 中选定的免疫因子和细胞/病毒 miRNA 的表达存在差异。一些 miRNA 和细胞因子参与病毒复制的控制、免疫反应/调节，可作为 ECs 诊断和与其他组区分的生物标志物。在 HIV 感染的不同阶段这些 miRNA 和细胞因子的差异表达有助于寻找新的感染控制方法。