MicroRNAs 谱分析在 HIV、HCV 和 HIV/HCV 合并感染患者中的应用。

MicroRNAs Profiling in HIV, HCV, and HIV/HCV Co-Infected Patients.

机构信息

Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Department of Microbiology, Faculty of Medicine, Fasa University of Medical Sciences, Fasa, Iran.

出版信息

Curr HIV Res. 2021;19(1):27-34. doi: 10.2174/1570162X18666200908112113.

DOI:10.2174/1570162X18666200908112113

PMID:32900348

Abstract

BACKGROUND

Human immunodeficiency virus (HIV) and hepatitis C virus (HCV) infections are important public health issues.

OBJECTIVE

This study aimed to assess the association between microRNAs expression leveland immunological and viral markers in HIV, HCV, and HIV/HCV co-infected patients.

METHODS

The expression level of miR-29, miR-149, miR-199, miR-let7, miR-223, miR-155, miR-122, and miR-150 was evaluated in 20 HIV, 20 HCV, 20 co-infected patients, and 20 healthy controls using real-time PCR assay. HIV and HCVviral loads were measuredby real-time PCR, and also, CD4+ T-lymphocyte count was measuredby the PIMA CD4 analyzer.

RESULTS

The miRNA expression pattern in each mentioned group showed significantly different expression profiles, but some miRNA species were shared between the groups. MiR-122 and miR-155 were upregulated, while miR-29 and miR-223 were downregulated in three patients groups compared to healthy controls. A significant positive correlation was observed between the expression of miR-122 and HIV/HCV loads. But, miR-29 and let-7 were negatively correlated with HIV load, and miR-149 and let-7 were negatively correlated with HCV load. Also, miR-155 was positively correlated with HCV load. MiR-122 and miR-199 were negative while others were positively correlated with CD4+ T cell count.

CONCLUSION

These miRNAs are probably involved in the clinical progression and pathogenesis of HIV and HCV infections. Therefore, determining and manipulating these miRNAs can lead to opening a new gate to control these important infections.

摘要

背景

人类免疫缺陷病毒（HIV）和丙型肝炎病毒（HCV）感染是重要的公共卫生问题。

目的

本研究旨在评估 HIV、HCV 和 HIV/HCV 合并感染患者中 microRNA 表达水平与免疫和病毒标志物之间的相关性。

方法

采用实时 PCR 法检测 20 例 HIV 患者、20 例 HCV 患者、20 例合并感染患者和 20 例健康对照者中 miR-29、miR-149、miR-199、miR-let7、miR-223、miR-155、miR-122 和 miR-150 的表达水平。采用实时 PCR 法检测 HIV 和 HCV 病毒载量，PIMA CD4 分析仪检测 CD4+T 淋巴细胞计数。

结果

各实验组 miRNA 表达模式显示出明显不同的表达谱，但有些 miRNA 种类在各组之间是共享的。与健康对照组相比，miR-122 和 miR-155 在三组患者中上调，而 miR-29 和 miR-223 下调。miR-122 的表达与 HIV/HCV 载量呈显著正相关。然而，miR-29 和 let-7 与 HIV 载量呈负相关，miR-149 和 let-7 与 HCV 载量呈负相关。此外，miR-155 与 HCV 载量呈正相关。miR-122 和 miR-199 呈负相关，而其他 miRNA 与 CD4+T 细胞计数呈正相关。